• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Molecular Biology Research >Divalent Cations Affect the Stability and Structure of Dad2p, a Subunit of the Candida Albicans Kinetochore Dam1 Complex
【24h】

Divalent Cations Affect the Stability and Structure of Dad2p, a Subunit of the Candida Albicans Kinetochore Dam1 Complex

机译：二价阳离子影响白色念珠菌Kinetochore Dam1复合体亚基Dad2p的稳定性和结构。

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The heterodecameric Dam1 complex is involved in establishing and maintaining the connection between the kinetochore and the mitotic spindle during mitosis. Biochemical studies of the reconstituted complex have shed light upon how it interacts with microtubules. However, little information about the biochemical properties of the isolated subunits has been available. This report examines the stability and structure of Dad2p, one of the Dam1 complex subunits isolated from Candida albicans. By employing differential scanning fluorimetry, protease protection and hydrodynamic analyses, we show that Dad2p is specifically responsive to the presence of divalent cations. This observation may be important for understanding the dynamic structure and regulation of the Dam1 complex in fungal cells.
机译：异十聚体Dam1复合物参与有丝分裂期间建立和维持线粒体和有丝分裂纺锤体之间的连接。重组复合物的生化研究揭示了其如何与微管相互作用。但是，关于分离的亚基的生化特性的信息很少。本报告检查了Dad2p的稳定性和结构，Dad2p是从白色念珠菌分离的Dam1复杂亚基之一。通过采用差示扫描荧光法，蛋白酶保护和流体力学分析，我们表明Dad2p专门响应二价阳离子的存在。该观察对于理解真菌细胞中Dam1复合物的动态结构和调控可能很重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号