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首页> 外文期刊>Journal of Molecular Genetics >Barcoding of Five Sturgeon Species in Iran
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Barcoding of Five Sturgeon Species in Iran

机译：伊朗五种St鱼的条形码

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摘要

The universal barcoding gene of Cytochrome Oxidase I (COI) was utilised for molecular characterisation of five sturgeon species found in the Iranian waters of the Caspian Sea. These were Acipenser gueldenstaedtii (Russian sturgeon), A. persicus (Persian sturgeon), A. stellatus (Stellate sturgeon), A. nudiventris (Spiny sturgeon) and Huso huso (Great sturgeon). An additional 10 sequences from the Genbank were included in the analysis, A. stellatus (AJ585050), H. huso (AY442351) A. baerii (FJ205560-FJ205563), A. fulvescens (EU524397), A. transmontanus (EU523891), A. medirostris (EU523885) and A. oxynrinchus (EU524401). The data was also analysed for phylogenetic insights among the studied species. The results confirmed the utility of this gene for molecular identification and revealed monophyly of three of the species; H. huso, A. nudiventris and A. stellatus. However, the status of A. gueldenstaedtii and A. persicus was unresolved with Genbank sequences of A. baerii (FJ205560-FJ205563) also clustering in the A. gueldenstaedtii-persicus complex as had been reported by previous researchers investigating other genes. Thus, the results provide further evidence of the polyphylic` nature of A. gueldenstaedtii. The data is also in agreement with several earlier reports that H. huso may not be a separate genus from Acipenser within the family Acipenserinae. The low internode bootstrap values at branch nodes did not allow for any phylogenetic inference among the Acipenser species to be deduced.
机译：细胞色素氧化酶I（COI）的通用条形码基因用于在里海伊朗海域发现的5种five鱼的分子表征。这些是Acipenser gueldenstaedtii（俄罗斯st），A。persicus（波斯st），A。stellatus（星状st），A。nudiventris（多刺st）和Huso huso（大st）。分析中还包括来自Genbank的另外10个序列，即拟南芥（AJ585050），胡氏梭状芽胞杆菌（AY442351）巴斯德拟南芥（FJ205560-FJ205563），富勒氏假单胞菌（EU524397），透山曲霉（EU523891），A medirostris（EU523885）和A. oxynrinchus（EU524401）。还对数据进行了分析，以研究物种之间的系统发育见解。结果证实了该基因在分子鉴定中的实用性，并揭示了其中三个物种的单亲性。 H. huso，A。nudiventris和A. stellatus。然而，正如先前研究其他基因的研究人员所报道的那样，百日咳杆菌的Genbank序列（FJ205560-FJ205563）无法解析百日咳杆菌和百日草的状态，百日咳杆菌的Genbank序列（FJ205560-FJ205563）也聚集在百日咳杆菌-persicus复合物中。因此，这些结果提供了进一步的证据，证明了A. gueldenstaedtii具有多系性。数据也与一些较早的报道一致，即胡氏梭菌可能不是Acipenserinae家族中与Acipenser分离的属。分支节点的节点间自举值较低，因此无法推断出Acipenser物种之间的任何系统发育推断。

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