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首页> 外文期刊>Journal of Molecular Genetics >Construction of a Vector Containing Coding Sequence of Lipid Transfer Proten-2 (LTP2) Gene from Rice
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Construction of a Vector Containing Coding Sequence of Lipid Transfer Proten-2 (LTP2) Gene from Rice

机译：水稻脂质转移Proten-2（LTP2）基因编码序列的载体构建

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摘要

Lipid Transfer Proteins (LTPs) are group of proteins with average molecular weight in plants that have potential applications especially in drug delivery systems. This research has been designed with the aim to construction of a vector containing coding sequence of Lipid Transfer Proten-2 (LTP2) gene from rice. There is no intron in rice LTP2 gene. In here, pGEX-6P-2 vector was used. First was extracted rice genome. Then, LTP2 gene was amplified with specific primers and PCR technique. In the next stage vector and LTP2 gene was digested with BamHI and XhoI restriction endonuclease enzymes. Then, digested vector and digested LTP2 gene were ligated in appropriate molar ratios.
机译：脂质转移蛋白（LTP）是植物中具有平均分子量的一组蛋白质，具有潜在的应用，尤其是在药物输送系统中。设计该研究的目的是构建包含水稻脂质转移Proten-2（LTP2）基因编码序列的载体。水稻LTP2基因中没有内含子。在这里，使用了pGEX-6P-2载体。首先是提取水稻基因组。然后，用特异性引物和PCR技术扩增LTP2基因。在下一阶段，将载体和LTP2基因用BamHI和XhoI限制性核酸内切酶消化。然后，将消化的载体和消化的LTP2基因以适当的摩尔比连接。

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