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首页> 外文期刊>Journal of Natural Sciences Research >Prevalence of microflora in trachea of NDV challenged broiler following supplementation with extracts of Aloe vera, Alma millsoni, Archachantina marginata and Ganoderma lucidum
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Prevalence of microflora in trachea of NDV challenged broiler following supplementation with extracts of Aloe vera, Alma millsoni, Archachantina marginata and Ganoderma lucidum

机译：补充芦荟提取物，阿拉木图提取物，侧生Archachantina边缘提取物和灵芝提取物后，NDV感染肉鸡的气管中微生物区系普遍存在

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An attempt was made to enumerate the micro-flora in trachea of broiler chickens challenged with Newcastle disease virus (NDV) following supplementation with different extracts of local sources. After 30 days of treatment with extracts of Aloe vera (AV), Alma millsoni (ALM), Archachatina marginata (AM) and G. lucidium (GL) ad libitum as supplement to various groups they were challenged with intramuscular administration of 0.2 ml of 106 ELD50 of NDV. Following clinical signs and symptoms in the birds, trachea swab samples were collected and cultured. Of the 95 swabs, 65 (68.4%) yielded mixed growth of bacteria spp. Pseudomonas aeruginosa (23.1%), Enterococcus feacalis (23.1%) and Staphylococcus spp. (23.1%), coliform bacteria (30.8%). Only 9 (13.9%) of the total population isolated resisted either cerofloxacin or genticin. There was no statistically significant difference (P<0.05) in the occurrences of bacteria in trachea of chickens supplemented with different concentration of extracts, both challenged with NDV and unchallenged groups. These organisms may not be pathogenic to the birds as there were no signs of bacterial infection on the trachea. Though, 86.1% of the isolates were sensitive to test drugs, evolution of strains resistant to common antibiotics by some of these bacteria could be of public health risk to handlers and the community. Thus, domestic birds keeping and poultry sited in close proximity to human dwellings should be outlawed.
机译：尝试补充不同来源的本地提取物后，对受到新城疫病毒（NDV）攻击的肉鸡气管中的菌群进行计数。用芦荟（AV），阿尔玛（Alm millsoni）（ALM），侧生Archachatina（AM）和G.lucidium（GL）的提取物作为各种组的补充剂治疗30天后，通过肌内注射0.2 ml的106进行攻击NDV的ELD50。根据鸟类的临床体征和症状，收集并培养气管拭子样本。在95个拭子中，有65个（68.4％）产生了细菌菌种混合生长。铜绿假单胞菌（23.1％），粪肠球菌（23.1％）和葡萄球菌属。 （23.1％），大肠菌（30.8％）。分离出的总种群中只有9（13.9％）抵抗头孢沙星或庆大霉素。补充了不同浓度提取物的鸡的气管内细菌的发生率均无统计学差异（P <0.05），无论是NDV攻击组还是未挑战组。这些生物可能对鸟类没有致病性，因为气管上没有细菌感染的迹象。尽管有86.1％的分离株对测试药物敏感，但其中一些细菌进化出对普通抗生素具有抗药性的菌株可能对操作人员和社区造成公共健康风险。因此，应禁止在人类住所附近饲养家禽和家禽。

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