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首页> 外文期刊>Journal of Molecular Biology Research >Effect of TiO2 Nanoparticles on Antioxidant Enzymes Activity and Biochemical Biomarkers in Pinto Bean (Phaseolus vulgaris L.)
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Effect of TiO2 Nanoparticles on Antioxidant Enzymes Activity and Biochemical Biomarkers in Pinto Bean (Phaseolus vulgaris L.)

机译：TiO2纳米颗粒对斑豆抗氧化酶活性及生化指标的影响

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Tests were done on the effects of titanium dioxide spray on Pinto bean (Phaseolus vulgaris L. c.v ‘c.o.s.16’). The study was conducted as a factorial experiment in a randomized complete block design with four replications for two years (2014 - 2015). Treatments consisted of two factors; the first factor was stage of plant growth that spraying was applied (rapid vegetative growth, flowering and pod filling); and the second factor was that of different concentrations of titanium dioxide nanoparticles (TiO2) that consisted of spray with water (control), nano titanium dioxide at concentrations of 0.01%, 0.02%, 0.03% and 0.05%. Activity of guaiacol peroxidase (GPX), activity of superoxide dismutase (SOD), activity of catalase (CAT), activity of peroxidase (POD), malonyldialdehyde (MDA) Content and 8-deoxy-2-hydroxyguanosine (8-OHDG) content were assayed. Results showed that effect of nano TiO2 was significant on activity of superoxide dismutase (SOD), activity of catalase (CAT), activity of peroxidase (POD), malonyldialdehyde (MDA) Content and 8-deoxy-2-hydroxyguanosine (8-OHDG) content. Results of combined analysis of variance showed that the effect year significantly affected on SOD and 8-OH-2-DG (P ≤ 0.05). The effect of different amounts of titanium dioxide nanoparticles (TiO2) significantly affected (P ≤ 0.05) on MDA and 8-OH-2-DG. The effects of different amounts of titanium dioxide nanoparticles and year were significant on SOD, POD, MDA and the amount of 8-deoxy-2-hydroxyguanosine in P ≤ 0.05. None of the physiological traits were affected by spraying of nano titanium dioxide. The effects of TiO2 nanoparticles times of spraying and year were significant on SOD, CAT and 8-deoxy-2-hydroxyguanosine (P ≤ 0.05). Interaction effects of nano TiO2 concentrations × nano TiO2 spraying times did not have a significant impact on SOD, CAT, POD, GPX, MDA and 8-OH-2-DG. Although, all trait were affected by interaction effects of year × nano TiO2 concentrations × nano TiO2 spraying times with the exception of GPX (P ≤ 0.05).
机译：测试了二氧化钛喷雾对斑豆（Phaseolus vulgaris L. c.v‘c.o.s.16’）的影响。这项研究是作为因子分解实验在随机完整区组设计中进行的，四年内重复四次（2014年至2015年）。治疗包括两个因素;第一个因素是施用喷雾的植物生长阶段（快速营养生长，开花和荚果填充）;第二个因素是不同浓度的二氧化钛纳米颗粒（TiO2），包括水喷雾（对照），浓度为0.01％，0.02％，0.03％和0.05％的纳米二氧化钛。愈创木酚过氧化物酶（GPX）的活性，超氧化物歧化酶（SOD）的活性，过氧化氢酶（CAT）的活性，过氧化物酶（POD）的活性，丙二醛（MDA）的含量和8-脱氧-2-羟基鸟苷（8-OHDG）的含量分析。结果表明，纳米TiO2对超氧化物歧化酶（SOD）活性，过氧化氢酶（CAT）活性，过氧化物酶（POD）活性，丙二醛（MDA）含量和8-脱氧-2-羟基鸟苷（8-OHDG）的影响显着。内容。方差组合分析的结果表明，影响年显着影响了SOD和8-OH-2-DG（P≤0.05）。不同量的二氧化钛纳米颗粒（TiO2）对MDA和8-OH-2-DG的影响显着（P≤0.05）。不同数量的二氧化钛纳米颗粒和年份对SOD，POD，MDA的影响以及8-deoxy-2-hydroxyguanosine的含量均具有显着性，P≤0.05。纳米二氧化钛的喷涂未影响任何生理特性。 TiO2纳米粒子的喷涂时间和年数对SOD，CAT和8-deoxy-2-hydroxyguanosine的影响显着（P≤0.05）。纳米TiO2浓度×纳米TiO2喷涂时间的相互作用影响对SOD，CAT，POD，GPX，MDA和8-OH-2-DG没有显着影响。尽管，除GPX外，所有性状均受年×纳米TiO2浓度×纳米TiO2喷涂时间的相互作用的影响（P≤0.05）。

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