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首页> 外文期刊>Journal of Molecular Biochemistry >Identification of drought-induced transcription factors in peanut (Arachis hypogaea L.)
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Identification of drought-induced transcription factors in peanut (Arachis hypogaea L.)

机译：鉴定干旱诱导的花生转录因子（Arachis hypogaea L.）

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Transcription factors play key roles in the regulation of genes involved in normal development as well as tolerance to biotic and abiotic stresses. Specific transcription factors that are induced in peanut under drought conditions have not been identified. The objectives of this study were to compare gene-expression patterns of various transcription factors of a drought tolerant versus a susceptible peanut genotype under drought conditions and to identify transcripts that were regulated in a drought dependent manner. Twelve putative transcription factors were identified and real-time PCR analysis was performed which resulted in the identification of three unique transcripts in which ahERF1 was highly induced in the recovery stage; ahERF7 and ahERF8 were also highly induced by drought and returned to nominal levels after recovery. These sequences contain DNA binding domains that are present in the APETALA2/Ethelene Responsive Factors (AP2/ERF) family of transcription factors which have been shown to be induced by stress. Induction levels and patterns of gene-expression of ahERF1, ahERF7 and ahERF8 may be used to select plants that may have higher drought tolerance.
机译：转录因子在调控参与正常发育的基因以及对生物和非生物胁迫的耐受性中起关键作用。尚未确定在干旱条件下在花生中诱导的特定转录因子。这项研究的目的是比较干旱条件下耐旱和易感花生基因型的各种转录因子的基因表达模式，并鉴定以干旱依赖性方式调控的转录本。鉴定了十二个推定的转录因子，并进行了实时PCR分析，鉴定了三个独特的转录本，其中在恢复阶段高度诱导了ahERF1。 ahERF7和ahERF8也受到干旱的强烈诱导，恢复后恢复到正常水平。这些序列包含存在于APETALA2 / Ethelene响应因子（AP2 / ERF）转录因子家族中的DNA结合结构域，这些结构域已显示受压力诱导。 ahERF1，ahERF7和ahERF8的诱导水平和基因表达模式可用于选择可能具有较高耐旱性的植物。

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