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首页> 外文期刊>Journal of neurological sciences (Turkish) >Radiation Induced TLR-8 Expression and TGF-β/TNF-α Production in BV2 Microglial Cells
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Radiation Induced TLR-8 Expression and TGF-β/TNF-α Production in BV2 Microglial Cells

机译：辐射诱导BV2小胶质细胞TLR-8表达和TGF-β/TNF-α的产生

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Objective: To observe the radiation-induced TLR-8 mRNA expression and production of proinflammatory cytokines in BV2 microglial cells.Methods: TLR-8 mRNA expression and distribution in BV-2 cells was analyzed by fluorescent quantitative PCR and flow measurement at different time point (1, 3, 6, 12, 24, 48 and 72 h) after a single 16 Gy dose of radiation, or at 24 h after various doses of radiation (2, 5, 10, 16, 20, 30 and 40 Gy).. ELISA was used to determinate the concentration of TNF-α and TGF-β in the supernatant of BV-2 cells.Results: The expression of TLR-8 mRNA in BV2 microglia cells was 0.000639 ± 0.00016, with 41.98% in the membrane and 32.08% in the cell. After 16 Gy radiation, the TLR-8 mRNA expression increased at 1h after radiation, with peak at 24 h. TLR-8 mRNA increased after 2 Gy radiation, and the value increased to top at 16 Gy. There was a steady increase in the concentration of TGF-β in the supernatant of BV2 microglial cells 48 h later. A slight effect on TNF-α was also found.Conclusion: The data suggests that TLR-8-induced activation of microglia and the release of TGF-β might be responsible for brain injury induced by radiation
机译：目的：观察辐射诱导的BV2小胶质细胞中TLR-8 mRNA的表达和促炎细胞因子的产生。方法：采用荧光定量PCR和流量测量法分析BV-2细胞中TLR-8 mRNA的表达和分布。在一次16 Gy剂量的辐射后（1、3、6、12、24、48和48小时），或在各种剂量的辐射（2、5、10、16、20、30和40 Gy之后的24小时）用ELISA法测定BV-2细胞上清液中TNF-α和TGF-β的浓度。结果：BV2小胶质细胞中TLR-8 mRNA的表达为0.000639±0.00016，膜中为41.98％。和32.08％在单元格中。放疗16 Gy后，放疗后1h TLR-8 mRNA表达增加，在24 h达到高峰。在2 Gy辐射后，TLR-8 mRNA升高，在16 Gy时，TLR-8 mRNA升高至最高。 48小时后，BV2小胶质细胞上清液中TGF-β的浓度稳定增加。结论：TLR-8诱导的小胶质细胞活化和TGF-β的释放可能是辐射引起的脑损伤的原因。

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