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首页> 外文期刊>Journal of oceanography >Laser-Dye Imaging of the pH Field in a Laboratory Experiment
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Laser-Dye Imaging of the pH Field in a Laboratory Experiment

机译：在实验室实验中对pH场进行激光染料成像

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We established a precise DeLIF (Dual Emission Laser Light Fluorescence) system for the instant measurement of the pH field in laboratory experiments. This method is very important in studies of the biological impacts of CO2 sequestration, i.e., effects of pH on organisms. The technique is demonstrated by applying it to the pH measurement around a liquid CO2 droplet, which has a large gradient of CO2 concentration near its interface, the concentration value on the boundary being known as the solubility of CO2. In the Laser Induced Fluorescence (LIF) method using quinine and an ultraviolet laser light source, the relatively unstable pulse of the laser source decreased the measurement reliability. We therefore investigated many kinds of fluorescent dye and demonstrated the DeLIF measurement, identifying a process for cancelling fluctuations of laser light intensity. Quinine and sulforhodamine were used as fluorescence dyes. The molar absorption coefficient and emission intensity of quinine depend on the pH of aqueous solutions, while those of sulforhodamine are not as much affected by pH. The ratio of these fluorescence intensities represents pH at each point of the image. As a result, the pH field was successfully measured with an uncertainty of only approximately ±0.005, for 3.0 < pH.
机译：我们建立了精确的DeLIF（双发射激光荧光）系统，用于在实验室实验中即时测量pH值。该方法在研究隔离CO2的生物学影响（即pH对生物的影响）中非常重要。通过将该技术应用于液态CO2液滴周围的pH测量来证明该技术，该液滴在其界面附近具有较大的CO2浓度梯度，边界上的浓度值称为CO2溶解度。在使用奎宁和紫外激光源的激光诱导荧光（LIF）方法中，激光源的相对不稳定的脉冲降低了测量的可靠性。因此，我们研究了多种荧光染料并演示了DeLIF测量，确定了消除激光强度波动的过程。奎宁和磺基罗丹明用作荧光染料。奎宁的摩尔吸收系数和发射强度取决于水溶液的pH值，而磺基若丹明的摩尔吸收系数和发射强度则不受pH值的影响。这些荧光强度的比率代表图像各点的pH。结果，对于3.0 H，成功测量了pH场，不确定度仅为±0.005。

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