• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oral Microbiology >In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health
【24h】

In-depth sequence analysis of highly-conserved pyrH gene to study distributions of oral treponemes in periodontal disease versus health

机译：高度保守的pyrH基因的深入序列分析，以研究牙周病与健康之间的口腔色氨酸分布

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: More than 75 species/species-level phylotypes of oral treponeme bacteria inhabit the oral cavity. However, their respective genomic compositions and clinical distributions remain poorly understood.Objectives: To compare distributions of phylogroup 1 and 2 oral treponemes in subjects with various periodontal health conditions, via sequence analysis of a highly-conserved treponeme ‘housekeeping’ gene.Methods: Subgingival plaque samples were collected from Chinese subjects with chronic periodontitis (n=5), aggressive periodontitis (n=4), gingivitis (n=5), and healthy controls (n=4). Samples were analyzed by a PCR/plasmid clone sequencing-based approach, using primer sets targeting the pyrH gene. Data was analyzed using various computational/bioinformatic approaches.Results: 1,227 quality-filtered pyrH gene sequences were obtained (mean 66.2±9.6 sequences per subject), which were assigned to 33 ‘pyrH genotypes’ (97% identity cut-off). 538 pyrH sequences (17 pyrH genotypes) corresponded to ...
机译：背景：口腔中存在着超过75种物种/种的系统，这是口腔类群的细菌。然而，它们各自的基因组组成和临床分布尚不清楚。从患有慢性牙周炎（n = 5），侵袭性牙周炎（n = 4），牙龈炎（n = 5）和健康对照（n = 4）的中国受试者中收集噬菌斑样品。使用靶向pyrH基因的引物组，通过基于PCR /质粒克隆测序的方法分析样品。结果：获得了1,227个质量过滤的pyrH基因序列（每个受试者平均66.2±9.6个序列），被分配给33个“ pyrH基因型”（97％同一性临界值）。 538个pyrH序列（17个pyrH基因型）对应于...

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号