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首页> 外文期刊>Journal of Oral Microbiology >Chronic Porphyromonas gingivalis infection accelerates the occurrence of age-related granules in ApoE–/– mice brains
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Chronic Porphyromonas gingivalis infection accelerates the occurrence of age-related granules in ApoE–/– mice brains

机译：慢性牙龈卟啉单胞菌感染会加速ApoE – / –小鼠大脑中与年龄有关的颗粒的发生

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摘要

This study explored the origin of age-related granules in the apolipoprotein E gene knockout (ApoE?/?) B6 background mice brains following chronic gingival infection with Porphyromonas gingivalis for 24?weeks. Intracerebral localization of P. gingivalis was detected by fluorescence in situ hybridization (FISH) and its protease by immunohistochemistry. The age-related granules were observed by periodic acid–Schiff (PAS), silver impregnation, and immunostaining. FISH showed intracerebral dissemination of P. gingivalis cells (p?=?0.001). PAS and silver impregnation demonstrated the presence of larger inclusions restricted to the CA1, CA2, and dentate gyrus sectors of the hippocampus. A specific monoclonal antibody to bacterial peptidoglycan detected clusters of granules with variable sizes in mice brains infected with P. gingivalis (p?=?0.004), and also highlighted areas of diffuse punctate staining equating to physical tissue damage. Mouse immunoglobulin G was observed in the capillaries of the cere...
机译：这项研究探讨了载脂蛋白E基因敲除（ApoEβ/β）B6背景小鼠大脑中慢性牙龈感染牙龈卟啉单胞菌24周后与年龄有关的颗粒的起源。通过荧光原位杂交（FISH）和其蛋白酶的免疫组织化学检测牙龈卟啉单胞菌的脑内定位。通过高碘酸席夫（PAS），银浸渍和免疫染色观察与年龄有关的颗粒。 FISH显示牙龈卟啉单胞菌细胞的脑内扩散（p≤0.001）。 PAS和银浸渍证明存在较大的夹杂物，仅限于海马的CA1，CA2和齿状回。一种针对细菌肽聚糖的特异性单克隆抗体可在感染牙龈卟啉单胞菌的小鼠大脑中检测出大小不等的颗粒簇（p≥0.004），并突出了点状弥散性染色区域，等同于物理组织损伤。在小鼠的毛细血管中观察到了小鼠免疫球蛋白G。

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