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首页> 外文期刊>Journal of Oral Microbiology >Metatranscriptomic analysis of an in vitro biofilm model reveals strain-specific interactions among multiple bacterial species
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Metatranscriptomic analysis of an in vitro biofilm model reveals strain-specific interactions among multiple bacterial species

机译：对体外生物膜模型的转录组分析显示了多种细菌之间的菌株特异性相互作用

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ABSTRACT Interactions among bacteria can affect biofilm properties. Method : Here, we investigated the role of different bacteria in functional dysbiosis of an in vitro polymicrobial subgingival plaque model using both 16S rRNA and metatranscriptomic sequencing. Results : We found that high-virulence Porphyromonas gingivalis W83 had greater effects on the symbiotic species than the low-virulence P. gingivalis ATCC33277, and that Prevotella intermedia exacerbated the effects of W83. P. gingivalis significantly influenced the expression of genes related to metabolic pathways and quorum sensing of commensal oral species in a strain-specific manner. P. intermedia exerted synergistic effects with P. gingivalis W83 but antagonistic effects with strain ATCC33277, which may regulate the expression of virulence factors of P. gingivalis through the clp regulator. Discussion : The interaction networks indicated that the strongest correlation was between Fusobacterium nucleatum and Streptococcus mitis , which demonstrated their bridge and cornerstone roles in biofilm. Changes in the expression of genes relating to outer membrane proteins in F. nucleatum indicated that the addition of different bacteria can interfere with the co-adherence among F. nucleatum and other partners. Conclusion : We report here the existence of strain-specific interactions in subgingival plaque, which may enhance our understanding of periodontal micro-ecology and facilitate the development of improved plaque control strategies.
机译：摘要细菌之间的相互作用会影响生物膜的特性。方法：在这里，我们使用16S rRNA和超转录组测序技术，研究了不同细菌在体外多菌性龈下菌斑模型功能失调中的作用。结果：我们发现，高毒力牙龈卟啉单胞菌W83比低毒力牙龈卟啉单胞菌ATCC33277对共生物种的影响更大，而中间型普氏杆菌加剧了W83的作用。牙龈卟啉单胞菌以菌株特异性方式显着影响与共生口腔物种的代谢途径和群体感应有关的基因的表达。中间假单胞菌与牙龈假单胞菌W83发挥协同作用，但与菌株ATCC33277产生拮抗作用，这可能通过clp调节剂调节牙龈假单胞菌毒力因子的表达。讨论：相互作用网络表明，核梭状芽孢杆菌和链球菌之间的相关性最强，这表明它们在生物膜中起着桥梁和基石的作用。核果外膜中与外膜蛋白有关的基因表达的变化表明，添加不同的细菌会干扰核果外膜与其他伴侣之间的共粘附。结论：我们在这里报告了龈下斑块中存在菌株特异性相互作用，这可能增进我们对牙周微生态学的理解并促进改进斑块控制策略的发展。

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