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首页> 外文期刊>Journal of physiology and pharmacology: an official journal of the Polish Physiological Society >LONG-TERM FEEDING WITH BIOACTIVE TRIPEPTIDES IN AGED HYPERTENSIVE AND NORMOTENSIVE RATS: SPECIAL FOCUS ON BLOOD PRESSURE AND BRADYKININ-INDUCED VASCULAR REACTIVITY
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LONG-TERM FEEDING WITH BIOACTIVE TRIPEPTIDES IN AGED HYPERTENSIVE AND NORMOTENSIVE RATS: SPECIAL FOCUS ON BLOOD PRESSURE AND BRADYKININ-INDUCED VASCULAR REACTIVITY

机译：老年高血压和非紧张性大鼠长期服用生物活性肽：对血压和缓激肽诱导的血管反应性的特别关注

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Bradykinin is the main player of the kallikrein-kinin system. Bradykinin-induced vasodilatation is age-dependent; this is believed to be associated with the level of expression of the two bradykinin receptors (BR1 and BR2) in the vasculature. The aim of this study was to clarify bradykinin-induced vascular reactivity of spontaneously hypertensive rats (SHR) and age-matched Wistar-Kyoto rats (WKY)after 6 weeks’ consumption of a drink containing bioactive tripeptides (Ile-Pro-Pro, Val-Pro-Pro and Leu-Pro-Pro). Two age groups were used: young (10 weeks at the end of experiment) and old (24 weeks). Blood pressure was monitored weekly by the tail-cuff method. After six weeks, vascular reactivity was assessed in vitro in mesenteric artery rings focusing on bradykinin induced activity. Blood pressure was lowered in old SHR after 6 weeks’ tripeptide consumption compared to water drinking controls (P <0.05). Blood pressure was lowered by peptide consumption also in old WKY (P < 0.05) but tripeptide consumption exerted no effect on the blood pressure of young animals. Old SHR suffered from endothelial and smooth muscle dysfunction which was not improved by these tripeptides. Interestingly,bradykinin caused vasoconstriction even in young SHR; this was blocked by a non-selective cyclooxygenase (COX) inhibitor but not by a B1 and B2 receptor antagonist. The expressions of mRNA of COX-1 and COX-2 in aorta were slightly upregulated in old SHR. ACE-1 activity in aorta and protein level in kidney, but not ACE-1 mRNA expression was upregulated in old animals (P < 0.05). To conclude,long-term feeding with a drink containing tripeptides lowers or prevents the age-associated increase in blood pressure in hypertensive and normotensive animals. ACE-1 activity, protein level but not mRNA expression are elevated in old animals. We also demonstrated that the vascular inflammation and dysfunction present in aged hypertensive animals cause bradykinin to induce vasoconstriction; this is not prevented by tripeptide feeding but involves the prostaglandin pathway.
机译：缓激肽是激肽释放酶激肽系统的主要参与者。缓激肽诱导的血管舒张是年龄依赖性的。据信这与脉动系统中两种缓激肽受体（BR1和BR2）的表达水平有关。这项研究的目的是澄清饮用含生物活性三肽的饮料（Ile-Pro-Pro，Val，6周后，自发性高血压大鼠（SHR）和年龄匹配的Wistar-Kyoto大鼠（WKY）缓激肽诱导的血管反应-Pro-Pro和Leu-Pro-Pro）。使用了两个年龄段：年轻人（实验结束时为10周）和老年人（24周）。每周通过尾袖法监测血压。六周后，在体外评估了缓激肽诱导的活性的肠系膜动脉环中的血管反应性。与饮水对照组相比，服用三周肽后6周，旧SHR的血压降低了（P <0.05）。在老年WKY中，肽的摄入也会降低血压（P <0.05），但是三肽的摄入对幼小动物的血压没有影响。老的SHR患有内皮和平滑肌功能障碍，而这些三肽并不能改善这种功能。有趣的是，缓激肽甚至在年轻的SHR中也引起血管收缩。它被非选择性环氧化酶（COX）抑制剂阻断，但未被B1和B2受体拮抗剂阻断。老年SHR患者主动脉COX-1和COX-2 mRNA的表达略有上调。在老年动物中，主动脉中的ACE-1活性和肾脏的蛋白水平上调，但ACE-1 mRNA的表达未上调（P <0.05）。总而言之，长期服用含三肽的饮料可以降低或预防与高血压和血压正常的动物年龄相关的血压升高。 ACE-1活性，蛋白质水平而非mRNA表达在老年动物中升高。我们还证明了在老年高血压动物中存在的血管炎症和功能障碍会导致缓激肽诱导血管收缩。三肽喂养不能防止这种情况，但是会涉及前列腺素途径。

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