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首页> 外文期刊>Journal of Pharmacy and Pharmaceutical Sciences >Co-encapsulation of a Drug with a Protein in Erythrocytes for Improved Drug Loading and Release: Phenytoin and Bovine Serum Albumin (BSA)
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Co-encapsulation of a Drug with a Protein in Erythrocytes for Improved Drug Loading and Release: Phenytoin and Bovine Serum Albumin (BSA)

机译：在红细胞中与蛋白质共包封药物以改善药物的装载和释放：苯妥英和牛血清白蛋白（BSA）

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The aim of the present study was to use a novel protein co-encapsulation method to prepare phenytoin-loaded human erythrocytes with improved loading parameters and release profiles. Carrier erythrocytes were prepared using the hypotonic pre-swelling method. A series of in vitro characterization tests were carried out on the carrier cells, including loading parameters, drug and hemoglobin release, hematological indices, particle size analysis, osmotic fragility, turbulence fragility, and scanning electron microscopy (SEM). Co-encapsulation with bovine serum albumin (BSA) resulted in about 8-times higher drug loading in erythrocytes, with biphasic release trend instead of triphasic in the case of drug alone loading. In comparison to the normal unloaded cells, MCH and MCHC indices were decreased in the case of both drug and drug/protein loading, apparent cell sizes were unchanged, cell shapes were changed to spherical rather than biconcave discoid, and the osmotic as well as turbulence fragilities were higher in the case of drug/protein but were unchanged in the case of drug alone loading. The most profound finding of this study was the possibility of achieving remarkably higher drug loading and more controllable drug release profile in the case of drug/protein loading, with no unwanted in vitro characteristics change.
机译：本研究的目的是使用一种新型的蛋白质共包封方法来制备具有改善的负载参数和释放曲线的苯妥英钠负载的人红细胞。使用低渗预溶胀法制备载体红细胞。在载体细胞上进行了一系列体外表征测试，包括负载参数，药物和血红蛋白释放，血液学指标，粒度分析，渗透脆性，湍流脆性和扫描电子显微镜（SEM）。与牛血清白蛋白（BSA）的共包封导致红细胞中的载药量提高了约8倍，在单独载药的情况下，具有双相释放趋势，而不是三相释放趋势。与正常的未加载细胞相比，在同时加载药物和药物/蛋白质的情况下，MCH和MCHC指数均降低，表观细胞大小未发生变化，细胞形状变为球形而不是双凹盘状，并且渗透和湍流在药物/蛋白质的情况下，脆性较高，但在单独装载药物的情况下，脆度没有变化。这项研究最深刻的发现是，在载有药物/蛋白质的情况下，可以获得显着更高的载药量和更可控的药物释放曲线，并且没有不希望的体外特征变化的可能性。

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