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首页> 外文期刊>Journal of Petroleum & Environmental Biotechnology >Detection and Quantification of Sulfate-Reducing and Polycyclic Aromatic Hydrocarbon-Degrading Bacteria in Oilfield Using Functional Markers and Quantitative PCR
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Detection and Quantification of Sulfate-Reducing and Polycyclic Aromatic Hydrocarbon-Degrading Bacteria in Oilfield Using Functional Markers and Quantitative PCR

机译：利用功能标记和定量PCR检测和定量测定油田中的硫酸盐还原和多环芳烃降解细菌

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Oilfield water samples from injection water treatment facility and soil/sludge samples from Gas Oil Separation Plant (GOSP) at Saudi Aramco were analyzed for the presence of SRB and PAH-degrading bacteria. SRB were detected by targeting a fragment of the apsA gene encoding adenosine-5-phosphosulfate reductase, which is characteristic of all SRB. The PAH-degrading bacteria were detected using a primer pair that amplifies a fragment of the gene encoding the large subunit of the naphthalene dioxygenase gene nahA. The nahA gene was detected in almost half of the soil/sludge samples with the highest copy number of 60540 copies/g soil/sludge. Most of the analyzed water samples contained high copy numbers of nahA gene with the highest copy number 3846 copies/ml. Most of the analyzed water samples revealed the presence of high copy numbers of the apsA gene with the highest copy number of 44 x 106/ml in sample number 2. Only 7 of the soil/sludge samples revealed the presence of the apsA gene with the highest copy number of 107920/g soil/sludge in sample number 11. In contrast to the nahA gene, the highest copy numbers of the apsA gene were detected in the water samples. SRB and PAH-degrading bacteria exist in some Saudi oilfields and appear to play a role in the H2S production and PAH degradation.
机译：分析了注入水处理设施中的油田水样和沙特阿美公司的瓦斯油分离厂（GOSP）的土壤/淤泥样中是否存在SRB和PAH降解细菌。通过靶向编码所有SRB特有的5磷酸腺苷还原酶的apsA基因片段来检测SRB。使用能扩增编码萘二加氧酶基因nahA大亚基的基因片段的引物对检测PAH降解细菌。在几乎一半的土壤/淤泥样品中检测到了nahA基因，其最高拷贝数为60540拷贝/ g土壤/淤泥。分析的大多数水样中含有高拷贝数的nahA基因，最高拷贝数为3846拷贝/ ml。大部分分析的水样品显示2号样品中存在高拷贝数的apsA基因，其最高拷贝数为44 x 106 / ml。只有7个土壤/污泥样品显示apsA基因存在并具有apsA基因。在11号样本中，最高拷贝数为107920 / g土壤/污泥。与nahA基因相反，在水样中检测到apsA基因的最高拷贝数。 SRB和PAH降解细菌存在于一些沙特油田中，似乎在H2S产生和PAH降解中起作用。

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