A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease

Padmanabhan P; Shanker R; Khanna P

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A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease

机译：油脂污染土壤中多环芳烃降解细菌的DNA提取和PCR检测方法

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摘要

A protocol for extraction of microbial DNA from oil-and grease-saturated soil is reported that can be used to target availability of specific genotypes of bacteria in extensively contaminated soils using the polymerase chain reaction. The methodology developed can be used in the biotreatibility studies for in situ bioremediation of hydrocarbon-contaminated sites.

机译：据报道，从油和油脂饱和的土壤中提取微生物DNA的协议可用于使用聚合酶链反应，针对广泛污染的土壤中细菌特定基因型的可用性。所开发的方法可用于生物处理性研究中，以进行烃污染位点的原位生物修复。

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《World Journal of Microbiology & Biotechnology》 |1998年第6期|共2页
作者
Padmanabhan P; Shanker R; Khanna P;
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原文格式 PDF
正文语种 eng
中图分类微生物学;
关键词
contamination; DNA; extraction; availability; bioremediation; genotypes; methodology; polymerase chain reaction; soil bacteria; biodegradation; diversity; aromatic hydrocarbons; oil spills; polluted soils; soil pollution; soil types; Ind;

机译：污染;DNA;提取;利用度;生物修复;基因型;方法;聚合酶链反应;土壤细菌;生物降解;多样性;芳烃;溢油;污染土壤;土壤污染;土壤类型;工业;

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专利

1. A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease [J] . Padmanabhan P, Shanker R, Khanna P World Journal of Microbiology & Biotechnology . 1998,第6期

机译：油脂污染土壤中多环芳烃降解细菌的DNA提取和PCR检测方法
2. Effects of Nonionic Surfactant Addition on Populations of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria in a Bioreactor Treating Contaminated Soil [J] . HONGBO ZHU, DAVID R. SINGLETON, MICHAEL D. AITKEN Environmental Science & Technology . 2010,第19期

机译：添加非离子表面活性剂对生物反应器处理污染土壤中多环芳烃降解菌种群的影响
3. Identifying polycyclic aromatic hydrocarbon-degrading bacteria in oil-contaminated surface waters at Deepwater Horizon by cultivation, stable isotope probing and pyrosequencing [J] . Tony Gutierrez Reviews in Environmental Science and Bio/Technology . 2011,第4期

机译：通过培养，稳定的同位素探测和焦磷酸测序鉴定深水地平线中被石油污染的地表水中的多环芳烃降解细菌
4. Study on Fluorescent Staining of Bacteria for Total Direct Counts in Polycyclic Aromatic Hydrocarbons (PAHs) Contaminated Soil with 5-(4,6-DichloroTriazin-2-yl) Amino Fluorescein (DTAF) [C] . Dan Zhang, Aizhong Ding, Lin Jiang, International Conference on Energy and Environmental Protection . 2013

机译：用5-（4,6-二氯噻嗪-2-基）氨基荧光素（DTAF）对多环芳烃（PAHS）污染土壤总直接计数的细菌荧光染色
5. Stable-isotope probing-based investigations of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated soil [D] . Jones, Maiysha D'ora 2010

机译：基于稳定同位素探测的土壤中多环芳烃降解细菌的研究
6. Effects of Nonionic Surfactant Addition on Populations of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria in a Bioreactor Treating Contaminated Soil [O] . Hongbo Zhu, David Singleton, Michael D. Aitken -1

机译：非离子表面活性剂的添加对在生物反应器中多环芳烃分解细菌种群的影响处理污染土壤
7. Solvent–water extraction method for the evaluation of polycyclic aromatic hydrocarbons bioavailability in coal–tar-contaminated soils [O] . P. H. Lee, K. P. Chao, S. K. Ong 2013

机译：溶剂 - 水提取方法，用于评价煤焦油污染土壤中的多环芳烃生物利用度

A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease

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