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首页> 外文期刊>Journal of Pharmacopuncture >Honey Bee Venom (Apis mellifera) Contains Anticoagulation Factors and Increases the Blood-clotting Time
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Honey Bee Venom (Apis mellifera) Contains Anticoagulation Factors and Increases the Blood-clotting Time

机译：蜜蜂毒液（Apis mellifera）含有抗凝血因子并增加了凝血时间

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Objectives: Bee venom (BV) is a complex mixture of proteins and contains proteins such as phospholipase and melittin, which have an effect on blood clotting and blood clots. The mechanism of action of honey bee venom (HBV, Apis mellifera) on human plasma proteins and its anti-thrombotic effect were studied. The purpose of this study was to investigate the anti-coagulation effect of BV and its effects on blood coagulation and purification. Methods: Crude venom obtained from Apis mellifera was selected. The anti-coagulation factor of the crude venom from this species was purified by using gel filtration chromatography (sephadex G-50), and the molecular weights of the anti-coagulants in this venom estimated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Blood samples were obtained from 10 rabbits, and the prothrombin time (PT) and the partial thromboplastin time (PTT) tests were conducted. The approximate lethal dose (LD) values of BV were determined. Results: Crude BV increased the blood clotting time. For BV concentrations from 1 to 4 mg/mL, clotting was not observed even at more than 300 seconds, standard deviations ; however, clotting was observed in the control group 13.8 s, . Thus, BV can be considered as containing anti-coagulation factors. Crude BV is composed 4 protein bands with molecular weights of 3, 15, 20 and 41 kilodalton (kDa), respectively. The of the crude BV was found to be . Conclusion: BV contains anti-coagulation factors. The fraction extracted from the Iranian bees contains proteins that are similar to anti-coagulation proteins, such as phospholipase and melittin, and that can increase the blood clotting times in vitro.
机译：目标：蜂毒（BV）是一种复杂的蛋白质混合物，含有诸如磷脂酶和蜂毒肽之类的蛋白质，对血液凝结和血凝块有影响。研究了蜜蜂毒液（HBV，蜜蜂）对人血浆蛋白的作用机理及其抗血栓形成作用。这项研究的目的是研究BV的抗凝作用及其对血液凝结和纯化的作用。方法：选择从蜜蜂蜜蜂获得的粗毒液。使用凝胶过滤色谱法（sephadex G-50）纯化该物种的粗毒液的抗凝因子，并使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS）估算该毒液中抗凝剂的分子量。 -页）。从10只兔子获得血液样品，并进行凝血酶原时间（PT）和部分凝血活酶时间（PTT）测试。确定了BV的近似致死剂量（LD）值。结果：粗BV增加了凝血时间。对于1至4 mg / mL的BV浓度，即使在超过300秒的标准偏差下也未观察到凝结。然而，对照组在13.8 s，观察到凝血。因此，BV可被认为含有抗凝因子。粗BV由4条蛋白带组成，分子量分别为3、15、20和41千道尔顿（kDa）。发现粗BV为。结论：BV含有抗凝血因子。从伊朗蜜蜂中提取的馏分包含与抗凝蛋白相似的蛋白，例如磷脂酶和蜂毒肽，并且可以增加体外凝血时间。

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