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首页> 外文期刊>Journal of Pharmacy and Pharmaceutical Sciences >A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....
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A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

机译：一种快速灵敏的HPLC方法，用于液体样品提取和紫外检测定量生物样品中的紫杉醇：紫杉醇负载型靶向聚合物胶束在药物动力学和组织分布研究中的应用.....

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A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of paclitaxel (PTX) in plasma, various organs and tumor tissues of tumor-bearing mice. Tissue specimens of liver, kidneys, spleen, lungs, heart and tumor were separately homogenized in normal saline. Plasma or tissue homogenate (250 μl) containing PTX and internal standard (diazepam) were extracted by diethyl ether (6 ml). The separation was achieved on a μ-Bondapak C 18 HPLC column using sodium acetate buffer solution (0.01 M)/acetonitrile (58/42 v/v) at pH 5 ± 0.1 and flow rate of 1.9 mL/min. The effluent was monitored at 227 nm and column temperature was adjusted at 58 o C. The internal standard and PTX were eluted at 4.2 and 5.2 min, respectively and no interfering peaks were observed. Calibration curves were linear over the concentration range of 0.25-10 μg/ml of PTX in plasma and 0.3-20 μg/ml PTX in tissue homogenates with acceptable precision and accuracy (<15%). The mean recoveries of the drug after plasma extraction was 87.4% ± 3.6 while those of tissue homogenates ranged from 62.1± 4.5 to 75.5± 3.2 depending on the type of tissues studied. PTX was stable in samples with no evidence of degradation during 3 freeze–thaw cycles and 3 months storage at ?70 °C. The developed HPLC method was applied to quantify PTX in the mouse plasma and tissues after intravenous administration of 10 mg equivalent PTX/Kg dose of PTX-loaded tocopherol succinate-chitosan-polyethylene glycol-folate (TS-CS-PEG-FA) micelles formulation or Anzatax ? (Cremophor ? EL- based formulation of PTX) to female Balb/c mice. This article is open to POST-PUBLICATION REVIEW . Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.
机译：开发了一种简单，快速，灵敏的反相HPLC方法，并已用于测定荷瘤小鼠血浆，各种器官和肿瘤组织中的紫杉醇（PTX）。肝，肾，脾，肺，心脏和肿瘤的组织标本分别在生理盐水中匀浆。用乙醚（6 ml）萃取含有PTX和内标（地西p）的血浆或组织匀浆（250μl）。在μ-BondapakC 18 HPLC色谱柱上使用乙酸钠缓冲溶液（0.01 M）/乙腈（58/42 v / v）在pH 5±0.1且流速为1.9 mL / min时完成分离。在227 nm处监测流出液，将色谱柱温度调节至58 oC。内标和PTX分别在4.2和5.2 min洗脱，没有观察到干扰峰。校准曲线在血浆中0.25-10μg/ ml PTX和组织匀浆中0.3-20μg/ ml PTX的浓度范围内呈线性，并且具有可接受的精度和准确度（<15％）。血浆提取后药物的平均回收率为87.4％±3.6，而组织匀浆的平均回收率则介于62.1±4.5至75.5±3.2之间，具体取决于研究的组织类型。 PTX在样品中稳定，在3个冻融循环中以及在？70°C下保存3个月没有降解的迹象。静脉内施用10 mg等量PTX / Kg剂量的PTX生育酚琥珀酸酯-壳聚糖​​-聚乙二醇-叶酸酯（TS-CS-PEG-FA）胶束制剂后，已开发的HPLC方法用于定量小鼠血浆和组织中的PTX还是Anzatax？ （基于Cremophor？EL的PTX配方）应用于雌性Balb / c小鼠。本文对POST-PUBLICATION REVIEW开放。已注册的读者（请参阅“针对读者”）可以通过在问题目录页面上单击摘要来发表评论。

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