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首页> 外文期刊>Journal of Pharmacopuncture >Study of Intravenous Single-Dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats
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Study of Intravenous Single-Dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

机译：Sprague-Dawley大鼠体内蟾蜍药物的静脉单剂量毒性试验研究

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Objectives: Bufonis venonum (BV) is toad venom and is the dried, white secretions of the auricular and the skin glands of toads. This study was performed to evaluate the toxicity of intravenous injection of Bufonis venonum pharmacopuncture (BVP) through a single-dose test with sprague-dawley (SD) rats. Methods: Twenty male and 20 female 6-week-old SD rats were injected intravenously in the caudal vein with BVP or normal saline. The animals were divided into four groups with five female and five male rats per group: the control group injected with normal saline, the low-dosage group injected with 0.1 mL/animal of BVP, the medium-dosage group injected with 0.5 mL/animal of BVP and the high-dosage group injected with 1.0 mL/animal of BVP. We performed clinical observations every day and body weight measurements on days 3, 7 and 14 after the injection. We also conducted hematology, serum biochemistry, and histological observations immediately after the observation period. Results: No mortalities were observed in any experimental group. Paleness occurred in the medium- and the high-dosage groups, and congestion on tails was observed in females in the medium- and the high-dosage groups. No significant changes in weight, hematology, serum biochemistry, and histological observations that could be attributed to the intravenous injection of BVP were observed in any experimental group. Conclusion: The lethal dose of intravenously-administered BVP in SD rats is over 1.0 mL/animal.
机译：目的：蟾蜍毒素（Bfonis venonum，BV）是蟾蜍的毒液，是蟾蜍耳廓和皮肤腺的干燥白色分泌物。这项研究的目的是通过对sprague-dawley（SD）大鼠进行单剂量试验来评估静脉注射Bufonis venonum Pharmaccopuncture（BVP）的毒性。方法：20只雄性和20只雌性6周龄SD大鼠在尾静脉内静脉注射BVP或生理盐水。将动物分成四组，每组五只雌性和五只雄性大鼠：对照组注射生理盐水，低剂量组注射0.1mL /动物的BVP，中剂量组注射0.5mL /动物的BVP和高剂量组分别注射1.0 mL /动物BVP。我们每天进行临床观察，并在注射后第3、7和14天进行体重测量。观察期结束后，我们还立即进行了血液学，血清生化和组织学观察。结果：在任何实验组中均未观察到死亡。在中等剂量和高剂量组中出现苍白，在中等剂量和高剂量组中的女性观察到尾巴充血。在任何实验组中，均未观察到体重，血液学，血清生物化学和组织学观察结果的显着变化，这些变化可归因于静脉注射BVP。结论：SD大鼠静脉注射BVP的致死剂量超过1.0 mL /动物。

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