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首页> 外文期刊>Journal of Pharmacopuncture >Intramuscular Single-dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats
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Intramuscular Single-dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats

机译：蟾蜍-丹利大鼠对蟾蜍药物的肌肉内单剂量毒性试验

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Objectives: Bufonis venonum (BV) is the dried white secretions of the auricular and skin glands of the toads Bufo bufo gargarizans or Bufo melanosticus Schneider. This study was performed to evaluate the toxicity of intramuscularly-administered Bufonis venonum pharmacopuncture (BVP) and to calculate its approximate lethality through a single-dose test with Sprague-Dawley (SD) rats. Methods: Twenty male and 20 female 6-week-old SD rats were injected intramuscularly with BVP or normal saline. The animals were divided into four groups with five female and five male rats per group: the control group injected with normal saline at 0.5 mL/animal, the low-dosage group injected with 0.125 mL/animal of BVP, the medium-dosage group injected with 0.25 mL/animal of BVP and the high-dosage group injected with 0.5 mL/animal of BVP. All injections were in the left thighs of the rats. After administration, we conducted clinical observations everyday and body weight measurements on days 3, 7 and 14 after the injection. We also carried out hematology, serum biochemistry, and histological observations on day 15 after treatment. Results: No mortalities were observed in any experimental group. No significant changes in weight, hematology, serum biochemistry, and histological observations that could be attributed to the intramuscular injection of BVP were observed in any experimental group. Conclusion: Lethal dose of BVP administered via intramuscular injection in SD rats is over 0.5 mL/animal.
机译：目的：蟾蜍（Bufonis venonum，简称BV）是蟾蜍或蟾蜍黑头蟾蜍的耳和皮肤腺的白色分泌物。进行这项研究的目的是评估肌肉注射Bufonis venonum药物针刺（BVP）的毒性，并通过对Sprague-Dawley（SD）大鼠进行单次剂量试验来计算其近似致死性。方法：20只雄性和20只雌性6周龄SD大鼠肌肉注射BVP或生理盐水。将动物分为四组，每组五只雌性和五只雄性大鼠：对照组注射0.5mL /动物的生理盐水，低剂量组注射0.125mL /动物的BVP，中等剂量组注射0.25毫升/动物的BVP和高剂量组注射0.5毫升/动物的BVP。所有注射都在大鼠的左大腿上。给药后，我们每天进行临床观察，并在注射后第3、7和14天进行体重测量。治疗后第15天，我们还进行了血液学，血清生化和组织学观察。结果：在任何实验组中均未观察到死亡。在任何实验组中，均未观察到体重，血液学，血清生化指标和肌内注射BVP引起的组织学改变。结论：SD大鼠肌肉注射BVP致死剂量超过0.5 mL /动物。

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