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首页> 外文期刊>Journal of Proteins and Proteomics >IMPACT OF FOOD AND FOLATE SUPPLEMENTATION DURING Salmonella TYPHI INFECTION IN Caenorhabditis elegans
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IMPACT OF FOOD AND FOLATE SUPPLEMENTATION DURING Salmonella TYPHI INFECTION IN Caenorhabditis elegans

机译：沙门氏菌感染期间食物和叶酸补充对秀丽隐杆线虫的影响

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摘要

Caenorhabditis elegans is an instructive and suitable model for studying pathogenesis of almost all humanpathogens. Salmonella Typhi is gram-negative facultative intracellular anaerobe that causes several patheticinfections. Necessary enriched nutrient ingestion during pathological conditions may reduce the harshness ofthe infection. We investigated the impact of folate and food supplementation during S. Typhi infection on themodel system, C. elegans. Our data indicated that folate supplementation (10 μg) increases the lifespan of S.Typhi infected C. elegans up to 20%. In combination with laboratory food source E. coli OP50, folate increases theinfected the worm’s lifespan to 40%. The wild type C. elegans infected by S. Typhi died with the LT50 of 60 ± 12 h.The LT50 of S. Typhi infected folt-1 mutant strain VC959 was 96 ± 6 h. However, the folate supplemented mutantworms exhibited an extended life with LT50 of 120 ± 6 h. The short time exposure and pharyngeal pumpingstudies confirmed that folt-1 mutant worm exhibited increased survival rate during pathogenic course at significantlevel when compared to wild-type. Our data revealed that folt-1 plays a significant role in host defense systemagainst S. Typhi infection and the folate supplementation in combination with food increases the host survivalduring S. Typhi infection.
机译：秀丽隐杆线虫是研究几乎所有人类病原体发病机理的一种指导性且合适的模型。伤寒沙门氏菌是革兰氏阴性兼性细胞内厌氧菌，可引起多种病理感染。在病理情况下摄入必要的丰富营养可减少感染的严重性。我们调查了鼠伤寒沙门氏菌感染期间叶酸和食物补充对模型系统秀丽隐杆线虫的影响。我们的数据表明，添加叶酸（10μg）可以将伤寒沙门氏菌感染的秀丽隐杆线虫的寿命提高20％。叶酸与实验室食物来源的大肠杆菌OP50相结合，可使感染蠕虫的寿命延长至40％。伤寒沙门氏菌感染的野生型秀丽隐杆线虫死亡，其LT50为60±12 h。伤寒沙门氏菌感染的folt-1突变株VC959的LT50为96±6 h。但是，补充叶酸的突变蠕虫的LT50为120±6小时，寿命更长。短时暴露和咽抽动研究证实，与野生型相比，folt-1突变蠕虫在致病过程中的存活率显着提高。我们的数据显示，folt-1在抵抗鼠伤寒沙门氏菌感染的宿主防御系统中起着重要作用，并且叶酸补充剂与食物的结合增加了鼠伤寒沙门氏菌感染期间宿主的存活率。

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