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首页> 外文期刊>Journal of Residuals Science & Technology >Effects of Abiotic Factors on Microbial Characteristics of Petroleum Contaminated Soils in Northern China
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Effects of Abiotic Factors on Microbial Characteristics of Petroleum Contaminated Soils in Northern China

机译：非生物因素对中国北方石油污染土壤微生物特性的影响

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In this study, the microbial community and the physico-chemical properties of soil samples from seven oil-fields in the north of China were analyzed using multiple methods. The study’s purpose was to analyze the influence of abiotic factors on the microbial activity and the biotic community. By using the Ultrasonic- Soxhle extraction method of hydrocarbon in oil composition were analyzed, and column chromatography combined with GC-MS was used to analyze the contaminant composition. The results showed that the highest oil concentrations in the soil reached 97.3 g/kg dry soil, which amounts to about 500–1,000 times higher than the background in uncontaminated soil. Moreover, the average nutrient content of contaminated soils was lower than the unpolluted controls. The total bacterial load in the soil was measured using the most probable number (MPN) method and the microbial activity was determined by fluorescein diacetate (FDA) activity. The results showed that soil contaminated with oil seriously affects and limits the microbial populations and their activities. The water content of the soil was the key limiting factor of the microbial populations and the FDA activity. With a water content of 6%, oil content below 15%, and a pH of 7.5–8.0, the conditions supported the highest levels of microbial growth and activity. Microbial populations were 10–100 times and the FDA activity was 2–5 times lower in samples originating from the arid Northwest of China compared to samples from Northwest China. In the northwestern region the microbial diversity was 3 times higher. The microbial communities were limited and suppressed whereby the local environment, the temperature, the water content and the oil content were the key factors that induced the biodiversity of the soil contaminated with oil in different analyses of the geography and the climate environment.
机译：本研究采用多种方法分析了中国北方七个油田土壤样品的微生物群落和理化性质。这项研究的目的是分析非生物因素对微生物活性和生物群落的影响。采用超声波-索氏萃取法分析了油中烃的组成，并采用柱色谱-GC-MS联用分析了污染物的组成。结果表明，土壤中最高的油含量达到97.3 g / kg干燥土壤，比未污染土壤中的本底高约500–1,000倍。此外，被污染土壤的平均养分含量低于未污染的对照。土壤中的总细菌负荷使用最可能数法（MPN）进行测量，微生物活性通过二乙酸荧光素（FDA）活性确定。结果表明，被油污染的土壤严重影响和限制了微生物种群及其活动。土壤的水分含量是限制微生物种群和FDA活性的关键因素。水分含量为6％，油含量低于15％，pH值为7.5-8.0，这些条件支持了最高水平的微生物生长和活性。与来自西北地区的样本相比，来自中国西北干旱地区的样本中的微生物种群是10–100倍，FDA活性低2–5倍。在西北地区，微生物多样性高出3倍。微生物群落受到限制和抑制，因此在不同的地理和气候环境分析中，当地环境，温度，水含量和油含量是导致受油污染的土壤生物多样性的关键因素。

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