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首页> 外文期刊>Journal of Plant Studies >The Effect of TDZ and 2, 4-D Concentrations on the Induction of Somatic Embryo and Embryogenesis in Different Cocoa Genotypes
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The Effect of TDZ and 2, 4-D Concentrations on the Induction of Somatic Embryo and Embryogenesis in Different Cocoa Genotypes

机译：TDZ和2，4-D浓度对不同可可基因型体细胞胚诱导和胚发生的影响

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The effect of 2, 4-D and TDZ concentrations on primary embryogenesis seem to be genotype dependent. There were significant differences between the control and the other treatments on somatic embryo production by genotype AMAZ 3-2. Genotype COCA 3370-5 did not show any significant difference among the treatments with 1mg/l 2, 4-D + 1μg/l TDZ, 1mg/l 2,4-D + 10μg/l TDZ and the control treatment. Treatment 2mg/l 2, 4-D + 10μg/l TDZ seem to be better for the production of somatic embryos by genotype COCA 3370-5. Lower concentrations of 2, 4-D + TDZ seem to generate greater proportions of normal somatic embryos as compared with higher 2,4-D + TDZ by genotypes AMAZ 3-2 and COCA3370-5 Somatic embryogenesis using floral tissue explants was used to induce primary somatic embryos from the staminodes of 16 cocoa genotypes. Four genotypes (AMAZ 3-2, AMAZ 10-1, COCA 3370-5 and GU 183H) showed high frequencies of embryogenesis while the remaining genotypes generated little or no embryos. Secondary embryos were induced from the cotyledonary explants of the four genotypes that exhibited efficient primary embryogenesis. Percentage embryogenesis generally increased with culture time. GU 183 which demonstrated the highest primary and secondary embryogenesis recorded the lowest conversion rate of somatic embryo into plantlets.
机译：2、4-D和TDZ浓度对原代胚胎发生的影响似乎与基因型有关。对照和其他处理在基因型AMAZ 3-2的体细胞胚产生上有显着差异。基因型COCA 3370-5在使用1mg / l 2、4-D +1μg/ l TDZ，1mg / l 2,4-D +10μg/ l TDZ的处理与对照处理之间没有显示任何显着差异。 2mg / l 2、4-D +10μg/ l TDZ处理似乎更适合通过基因型COCA 3370-5生产体细胞胚。与AMAZ 3-2和COCA3370-5基因型较高的2,4-D + TDZ相比，较低浓度的2,4-D + TDZ似乎会产生更大比例的正常体细胞胚，使用花组织外植体诱导体细胞胚发生来自16种可可基因型的茎的初级体细胞胚。四种基因型（AMAZ 3-2，AMAZ 10-1，COCA 3370-5和GU 183H）显示出高频率的胚发生，而其余基因型几乎不产生胚胎。从表现出有效的初级胚胎发生的四种基因型的子叶外植体诱导次级胚。胚胎发生百分比通常随培养时间而增加。证明最高一级和二级胚胎发生的GU 183记录了体胚转化为小植株的转化率最低。

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