...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of reproduction and fertility >Distribution of lysophospholipids and metabolism of platelet-activating factor in human follicular and peritoneal fluids
【24h】

Distribution of lysophospholipids and metabolism of platelet-activating factor in human follicular and peritoneal fluids

机译：人卵泡和腹膜液中溶血磷脂的分布和血小板活化因子的代谢

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The distribution of lysophosphatidylcholine, lyso-platelet-activating factor and platelet-activating factor (PAF) was studied in human plasma and in follicular and peritoneal fluid. In plasma, peritoneal and follicular fluids, 51%, 87% and 89%, respectively, of the total lipids were found in the protein fraction (the density > 1.21 fraction). Two forms of lyso-phospholipids were identified in this fraction: one of high affinity and one of low affinity for albumin. The metabolism of PAF in human follicular fluid, peritoneal fluid and plasma was also investigated. PAF-acetylhydrolase activity was found in both peritoneal and follicular fluids which induced a time-dependent hydrolysis of [3H]PAF. The half-life of PAF was estimated to be 7–12 min in plasma, 15–25 min in peritoneal fluid and approximately 2 h in follicular fluid. PAF-acetylhydrolase activity in embryo culture media supplemented with 10% serum was markedly inhibited by addition of commercial serum albumin. When 25 g albumin l?1 was added, 22% of [3H]PAF was hydrolysed h?1 compared with 72% in media without albumin. The concentrations of lysophosphatidylcholine measured in plasma, in follicular and peritoneal fluids were 252, 286 and 53 μmol l?1, respectively. The distribution of these lysophospholipids and the metabolism of PAF in the female genital tract fluids reported in the present study provide evidence for the involvement of these biologically active lipid mediators in a variety of reproductive processes including sperm–egg interactions and embryonic development.
机译：研究了血浆，卵泡和腹膜液中溶血磷脂酰胆碱，溶血血小板活化因子和血小板活化因子（PAF）的分布。在血浆，腹膜和卵泡液中，在蛋白质级分中发现总脂质的比例分别为51％，87％和89％（密度> 1.21级分）。在该级分中鉴定出两种形式的溶血磷脂：一种对白蛋白具有高亲和力，一种对白蛋白具有低亲和力。还研究了人卵泡液，腹膜液和血浆中PAF的代谢。在腹膜和卵泡液中均发现了PAF-乙酰水解酶的活性，这引起了[3H] PAF的时间依赖性水解。 PAF的半衰期在血浆中估计为7–12分钟，在腹膜液中为15–25分钟，在卵泡液中大约为2 h。添加商业血清白蛋白可显着抑制补充了10％血清的胚胎培养基中的PAF-乙酰水解酶活性。当加入25克白蛋白l-1时，22％的[3H] PAF被h-1水解，而在没有白蛋白的培养基中则为72％。血浆，卵泡液和腹膜液中溶血磷脂酰胆碱的浓度分别为252、286和53μmoll？1。在本研究中报道的这些溶血磷脂的分布和女性生殖道液中PAF的代谢为这些生物活性脂质介体参与了多种生殖过程（包括精卵相互作用和胚胎发育）提供了证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号