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首页> 外文期刊>Journal of reproduction and fertility >Effects of luteotrophic and luteolytic hormones on expression of mRNA encoding insulin-like growth factor I and growth hormone receptor in the ovine corpus luteum
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Effects of luteotrophic and luteolytic hormones on expression of mRNA encoding insulin-like growth factor I and growth hormone receptor in the ovine corpus luteum

机译：营养营养和溶血激素对黄体中胰岛素样生长因子I和生长激素受体mRNA表达的影响

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The regulation of mRNAs encoding insulin-like growth factor I (IGF-I) and the receptor for growth hormone (GH-R) in ovine luteal tissue by luteotrophic and luteolytic hormones was examined. In Expt 1, ewes were hypophysectomized (HPX) on day 5 of the oestrous cycle and administered saline (S), LH, GH, or LH + GH until day 12 of the oestrous cycle (n = 4 ewes per group). Concentrations of luteal mRNA encoding IGF-I in HPX + S ewes and pituitary-intact ewes at day 5 (n = 4) were approximately 60% (P < 0.05) of those in pituitary-intact ewes at day 12 (n = 4). Treatment of HPX ewes with GH or GH + LH, but not LH alone, increased concentrations of mRNA encoding IGF-I to values similar to those in pituitary-intact ewes at day 12. Hypophysectomy also reduced the mean concentration of mRNA encoding GH-R to approximately 60% (P < 0.05) of the values in pituitary-intact ewes (days 5 or 12). Treatment with LH, but not GH, increased (P < 0.05) concentrations of mRNA encoding GH-R to values observed in pituitary-intact ewes. In Expt 2, prostaglandin F2伪 (PGF2伪; 1 渭mole) injected into the ovarian artery on day 11 or day 12 of the oestrous cycle had no effect on luteal concentrations of mRNA for either IGF-I or GH-R. In Expt 3, concentrations of mRNA encoding IGF-I increased (P < 0.05) between days 3 and 6 and remained high for the duration (days 9, 12 and 15) of the oestrous cycle while luteal concentrations of mRNA encoding GH-R did not change. In conclusion, responsiveness of the corpus luteum to GH and luteal synthesis of IGF-I are likely regulators of luteal development and function. However, PGF2伪-induced luteolysis was not associated with a decrease in concentrations of mRNAs encoding either IGF-I or GH-R.
机译：研究了黄体营养和黄体溶解激素对绵羊黄体组织中编码胰岛素样生长因子I（IGF-1）和生长激素受体（GH-R）的mRNA的调节作用。在Expt 1中，在雌性周期的第5天对母羊进行hypophytomecttomized（HPX）并给予生理盐水（S），LH，GH或LH + GH直到雌性周期的第12天（每组n = 4头母羊）。在第5天（n = 4），HPX +母羊和垂体功能完好的母羊中编码IGF-I的黄体mRNA的浓度约为第12天在垂体功能完好的母羊中的60％（P <0.05）（n = 4）。用GH或GH + LH（但不单独使用LH）处理HPX母羊时，编码IGF-I的mRNA浓度增加至与垂体完整的母羊在第12天时相似的水平。垂体切除术还降低了编码GH-R的mRNA的平均浓度至垂体完整母羊（第5天或第12天）的值的大约60％（P <0.05）。用LH而不是GH进行的处理将编码GH-R的mRNA的浓度增加（P <0.05）至在垂体完整的母羊中观察到的值。在实验2中，在雌性周期的第11天或第12天注射入卵巢动脉的前列腺素F2α（PGF2α;1μmol）对IGF-1或GH-R的黄体mRNA浓度没有影响。在Expt 3中，编码IGF-I的mRNA的浓度在第3天和第6天之间增加（P <0.05），并在发情周期的持续时间（第9、12和15天）保持较高，而黄体中编码GH-R的mRNA的浓度没变。总之，黄体对GH的反应性和黄体合成的IGF-1可能是黄体发育和功能的调节剂。但是，PGF2α诱导的黄体溶解与编码IGF-1或GH-R的mRNA浓度降低无关。

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