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首页> 外文期刊>Journal of the Brazilian Chemical Society >The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method
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The violacein biosynthesis monitored by multi-wavelength fluorescence spectroscopy and by the PARAFAC method

机译：通过多波长荧光光谱法和PARAFAC方法监测紫罗兰素的生物合成

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Obtaining information about a biosynthetic pathway is a complex and laborious procedure. In this sense, this work presents a new approach for the initial analysis of the biosynthesis of fluorescent natural products using as example the violacein biosynthesis. For this, a culture of Chromobacterium violaceum was grown in a bioreactor from which aliquots were collected every 2 h for subsequent analysis by multi-wavelength fluorescence spectroscopy. The excitation-emission matrices demonstrated the dynamic behavior of the fluorophores signal that are consumed and produced by the bacterium. These signals were resolved by PARAFAC (parallel factor analysis) method totalizing six pure components. Tryptophan and violacein were identified by comparison to spectra available in the literature. The identification of other fluorophores was critical step due to the lack of a database of fluorescent natural products to compare spectra. Finally, this methodology has great potential to achieve a deeper insight into the biosynthesis of natural products.
机译：获得有关生物合成途径的信息是一个复杂而费力的过程。从这个意义上讲，这项工作提出了一种新的方法，以荧光素天然生物合成为例，初步分析荧光天然产物的生物合成。为此，在生物反应器中培养紫罗兰色杆菌培养物，每2小时从中收集等分试样，用于随后的多波长荧光光谱分析。激发-发射矩阵证明了细菌消耗和产生的荧光团信号的动态行为。这些信号通过PARAFAC（并行因子分析）方法解析，总计六个纯组分。通过与文献中可用的光谱进行比较来鉴定色氨酸和紫罗兰素。由于缺乏用于比较光谱的荧光天然产物的数据库，其他荧光团的鉴定是关键步骤。最后，这种方法具有巨大的潜力，可以更深入地了解天然产物的生物合成。

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