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首页> 外文期刊>Journal of Veterinary Advances >Monitoring of GMO's in Livestock Feeds, Feed Stuffs and Products using PCR based Micro Array
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Monitoring of GMO's in Livestock Feeds, Feed Stuffs and Products using PCR based Micro Array

机译：使用基于PCR的微阵列监测家畜饲料，饲料原料和产品中的转基因生物

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It is vital for the developing countries to regulate GMO's with primary intention to protect consumer health, environment and at the time to reap the benefits of modern technology. Monitoring implementation of GMO regulatory frame work may be difficult in developing countries due to lack of resources and cost of post - release monitoring of GMO's. However strategies can be planned to overcome the same. India is the one of the largest exporters of non GMO foods. Indian livestock activity has boomed into an industry and the products are being exported. With WTO implementation the food produced needs to be labelled -GMO / Non GMO food. A monitoring programme has been initiated for screening and identifying GMO's in various livestock feeds, feedstuff and products under the aegis of Drugs & Pharmaceuticals Research Programme of DST (Government of India). The techniques are based on the detection of multiple genetic elements common to GMO by their amplification via PCR followed by direct hybridsation of the amplicons on Microarray. The experiment is composed of three sets of multiplex chain reaction (PCR) using biotinylated primers followed by hybridization of the biotinylated amplicons on one DNA Microarray. The detection of the hybridized biotinylated amplicons is performed using the silverquant colorimetric detection. The pattern of the elements is compared to a database of the composition of European/FDA approved GMO's and identification of the GMO is then proposed. The limit of detection of the method was ≤ 0.1% GMO content (w/w) expressed as the amount of target DNA present in the template for single unprocessed material. The DNA targets were detected both in reference material and in mixtures with same detection limit. The protocol of sampling method (EC 1830/2003) and the results will be elaborated. The profile revealed the cotton seed cake to be positive while all the other feeds and feed stuff not detected.
机译：对于发展中国家而言，至关重要的是监管转基因生物，其主要目的是保护消费者的健康，环境，并在当时获得现代技术的好处。由于缺乏资源和对转基因生物进行发布后监测的成本，在发展中国家可能难以监测转基因生物监管框架的执行情况。但是，可以计划采取策略来克服同样的情况。印度是非转基因食品的最大出口国之一。印度的畜牧业已发展成为一个行业，产品正在出口。随着WTO的实施，生产的食品需要贴上-GMO /非GMO食品标签。在DST（印度政府）的药品研究计划的主持下，已经启动了一项监测计划，以筛查和鉴定各种牲畜饲料，饲料和产品中的转基因生物。该技术基于通过PCR扩增GMO共同的多个遗传元件，然后在微阵列上直接扩增这些扩增子进行检测。该实验由三组使用生物素化引物的多重链式反应（PCR）组成，然后在一个DNA微阵列上杂交生物素化扩增子。杂交的生物素化的扩增子的检测使用银定量比色检测进行。将元素的图案与欧洲/ FDA批准的GMO组成的数据库进行比较，然后提出对GMO的鉴定。该方法的检出限为≤0.1％GMO含量（w / w），表示为模板中存在的单个未加工材料的目标DNA量。在目标物质和混合物中均以相同的检测限检测到了DNA靶标。将详细说明采样方法的协议（EC 1830/2003）和结果。概况显示棉籽饼呈阳性，而所有其他饲料和饲料原料均未检出。

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