• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Veterinary Advances >Anti-trypanosomal Activity of Piper Nigrum L (Black pepper) Against Trypanosoma Evansi
【24h】

Anti-trypanosomal Activity of Piper Nigrum L (Black pepper) Against Trypanosoma Evansi

机译：黑胡椒对锥虫的抗锥虫活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In search of antitrypanosomal compounds from medicinal plant, dried fruits of Piper nigrum (Black pepper) were extracted with methanolic solvent at concentrations (250-1000 μg ml-1). The methanolic plant extract (MPE) obtained was tested against Trypanosoma evansi for trypanocidal activity. This was carried out on Vero cells grown in Dulbecco's Modified Eagle Medium (DMEM) and supplemented with foetal calf serum (FCS) 20-40% at appropriate conditions. In vitro cytotoxicity test of P, nigrum fruits extract at concentrations (1.56-100 μg ml-1) was done on Vero cells but without FCS. In vitro trypanocidal activity varied from immobilization, reduction and to the killing of trypanosomes in corresponding ELISA plate wells. At 750 μg ml-1of MPE, there was reduction of average mean trypanosomes count in extract as observed. At 1000 μg ml-1 of the test extract of P. Nigrum , there was complete killing of trypanosomes at 7 h of incubation. Trypanosomes counts decreased in concentration and time –dependent manner with significant difference (P<0.05). MPE of P, nigrum and diminazine aceturate, standard drug, were cytotoxic to Vero cells except at concentrations of 1.56-3.13 and 1.56-6. 25 μg ml-1. Moderate anti-trypanosmal activity of MPE of P. nigrum was observed.
机译：为了从药用植物中寻找抗锥虫化合物，用浓度为250-1000μgml-1的甲醇溶剂提取黑胡椒的干果。测试获得的甲醇植物提取物（MPE）对抗伊万氏锥虫的锥虫杀虫活性。这是对在Dulbecco改良Eagle培养基（DMEM）中生长并在适当条件下补充20-40％的胎牛血清（FCS）的Vero细胞进行的。在没有FCS的Vero细胞上进行了浓度为（1.56-100μgml-1）的黑胡椒果实提取物的体外细胞毒性测试。在相应的ELISA板孔中，体外锥虫杀灭活性从固定，减少到杀死锥虫的过程有所不同。在750μgml-1的MPE中，观察到提取物中锥虫的平均平均计数减少。在1000μgml-1的P. Nigrum的测试提取物中，在孵育7小时后，锥虫小体被完全杀死。锥虫计数的浓度和时间依赖性降低，差异有统计学意义（P <0.05）。除浓度为1.56-3.13和1.56-6以外，标准药物P，黑药和醋酸二嗪嗪的MPE对Vero细胞具有细胞毒性。 25微克ml-1。观察到了黑僵菌MPE的中等抗锥虫活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号