...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Tissue Engineering >The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds
【24h】

The fusion of tissue spheroids attached to pre-stretched electrospun polyurethane scaffolds

机译：组织球体与预拉伸电纺聚氨酯支架的融合

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Effective cell invasion into thick electrospun biomimetic scaffolds is an unsolved problem. One possible strategy to biofabricate tissue constructs of desirable thickness and material properties without the need for cell invasion is to use thin (<2 μm) porous electrospun meshes and self-assembling (capable of tissue fusion) tissue spheroids as building blocks. Pre-stretched electrospun meshes remained taut in cell culture and were able to support tissue spheroids with minimal deformation. We hypothesize that elastic electrospun scaffolds could be used as temporal support templates for rapid self-assembly of cell spheroids into higher order tissue structures, such as engineered vascular tissue. The aim of this study was to investigate how the attachment of tissue spheroids to pre-stretched polyurethane scaffolds may interfere with the tissue fusion process. Tissue spheroids attached, spread, and fused after being placed on pre-stretched polyurethane electrospun matrices and formed tissue constructs. Efforts to eliminate hole defects with fibrogenic tissue growth factor-β resulted in the increased synthesis of collagen and periostin and a dramatic reduction in hole size and number. In control experiments, tissue spheroids fuse on a non-adhesive hydrogel and form continuous tissue constructs without holes. Our data demonstrate that tissue spheroids attached to thin stretched elastic electrospun scaffolds have an interrupted tissue fusion process. The resulting tissue-engineered construct phenotype is a direct outcome of the delicate balance of the competing physical forces operating during the tissue fusion process at the interface of the pre-stretched elastic scaffold and the attached tissue spheroids. We have shown that with appropriate treatments, this process can be modulated, and thus, a thin pre-stretched elastic polyurethane electrospun scaffold could serve as a supporting template for rapid biofabrication of thick tissue-engineered constructs without the need for cell invasion.
机译：有效的细胞侵入厚的电纺丝仿生支架中是尚未解决的问题。生物制造具有所需厚度和材料特性的组织构造而不需要细胞入侵的一种可能策略是使用薄的（<2μm）多孔电纺网和自组装（能够组织融合）的组织球体作为构建基块。预拉伸的电纺网在细胞培养中仍然绷紧，并且能够以最小的变形支撑组织球体。我们假设弹性电纺丝支架可以用作暂时的支持模板，以将细胞球体快速自组装成高级组织结构，例如工程化的血管组织。这项研究的目的是研究组织球体对预拉伸聚氨酯支架的附着如何干扰组织融合过程。在放置在预拉伸的聚氨酯电纺丝基质上并形成组织构造体后，组织球体附着，扩散并融合。用纤维化组织生长因子-β消除孔缺陷的努力导致胶原蛋白和骨膜蛋白的合成增加，并且孔的大小和数量显着减少。在对照实验中，组织球体融合在非粘性水凝胶上，形成没有孔的连续组织构造。我们的数据表明，附着在细长的弹性电纺丝支架上的组织球体的组织融合过程中断。所得的组织工程构造表型是在预拉伸的弹性支架和附着的组织球体的界面处在组织融合过程中运行的竞争性物理力的微妙平衡的直接结果。我们已经表明，通过适当的处理，可以调节该过程，因此，薄的预拉伸弹性聚氨酯电纺丝支架可以作为支持模板，用于快速生物制造厚组织工程构造，而无需细胞入侵。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号