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首页> 外文期刊>Journal of Seed Science >Etiology and pathogenicity of two different isolates of Colletotrichum spp. obtained from physic nut seeds
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Etiology and pathogenicity of two different isolates of Colletotrichum spp. obtained from physic nut seeds

机译：两种炭疽菌分离株的病原学和致病性。从物理坚果种子获得

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The species known as physic nut (Jatropha curcas L.) has become important as one of main sources of feedstock for biodiesel production. The aims of this study were characterizing two different isolates of Colletotrichum spp. obtained from seeds of this species, through morphological, cultural, and molecular analyses; as well as assessing pathogenicity of both isolates on leaves and fruit of this plant species. For morphological analysis, length and width of 30 spores of each isolate, produced on medium Malt Extract-Agar (MEA), at 25 ± 1 oC, and under constant light, were measured. Cultural analysis was performed by the fungus growth on PDA medium, through daily measurements of the mycelial growth, and the color of colonies after seven days incubation. DNA of the isolates was extracted and specific oligonucleotides primers (region ITS4) were identified by PCR and used to identify C. capsici (CcInt) and C. gloeosporioides (CgInt). Pathogenicity of isolates was assessed on plants aged 10, 20, 30, 40, 50, and 60 days after sowing and fruits at six maturation stages. Based on these analyses, isolates were identified as C. gloeosporioides and C. capsici. Young plants and the older fruits were more susceptible to infection by the two fungus species.
机译：作为生物柴油的主要原料来源之一，被称为物理坚果（麻疯树）的物种已变得很重要。这项研究的目的是表征炭疽菌的两个不同分离株。通过形态，文化和分子分析从该物种的种子获得;以及评估这两种分离物在该植物物种的叶片和果实上的致病性。为了进行形态学分析，测量了在25±1 oC且在恒定光照下在中等麦芽浸提琼脂（MEA）上产生的每种分离物的30个孢子的长度和宽度。通过在PDA培养基上真菌生长，每天测量菌丝体生长以及孵育7天后菌落的颜色来进行培养分析。提取分离物的DNA，并通过PCR鉴定特异性寡核苷酸引物（ITS4区），并用于鉴定辣椒衣壳梭菌（CcInt）和球孢梭菌（CgInt）。在六个成熟阶段播种后10、20、30、40、50和60天的植物和果实上评估了分离株的致病性。基于这些分析，分离物被鉴定为球孢梭菌和辣椒梭菌。年轻的植物和较老的果实更容易被这两种真菌感染。

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