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首页> 外文期刊>Journal of Tissue Engineering >Calcium phosphate thin films enhance the response of human mesenchymal stem cells to nanostructured titanium surfaces
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Calcium phosphate thin films enhance the response of human mesenchymal stem cells to nanostructured titanium surfaces

机译：磷酸钙薄膜增强人间充质干细胞对纳米结构钛表面的反应

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The development of biomaterial surfaces possessing the topographical cues that can promote mesenchymal stem cell recruitment and, in particular, those capable of subsequently directing osteogenic differentiation is of increasing importance for the advancement of tissue engineering. While it is accepted that it is the interaction with specific nanoscale topography that induces mesenchymal stem cell differentiation, the potential for an attendant bioactive chemistry working in tandem with such nanoscale features to enhance this effect has not been considered to any great extent. This article presents a study of mesenchymal stem cell response to conformal bioactive calcium phosphate thin films sputter deposited onto a polycrystalline titanium nanostructured surface with proven capability to directly induce osteogenic differentiation in human bone marrow–derived mesenchymal stem cells. The sputter deposited surfaces supported high levels of human bone marrow–derived mesenchymal stem cell adherence and proliferation, as determined by DNA quantification. Furthermore, they were also found to be capable of directly promoting significant levels of osteogenic differentiation. Specifically, alkaline phosphatase activity, gene expression and immunocytochemical localisation of key osteogenic markers revealed that the nanostructured titanium surfaces and the bioactive calcium phosphate coatings could direct the differentiation towards an osteogenic lineage. Moreover, the addition of the calcium phosphate chemistry to the topographical profile of the titanium was found to induce increased human bone marrow–derived mesenchymal stem cell differentiation compared to that observed for either the titanium or calcium phosphate coating without an underlying nanostructure. Hence, the results presented here highlight that a clear benefit can be achieved from a surface engineering strategy that combines a defined surface topography with an attendant, conformal bioactive chemistry to enhance the direct osteogenic differentiation of human bone marrow–derived mesenchymal stem cells.
机译：具有可促进间充质干细胞募集的地形学线索的生物材料表面的发展，特别是那些能够随后指导成骨细胞分化的生物材料表面，对于组织工程的发展越来越重要。尽管与特定纳米级地形的相互作用引起了间充质干细胞的分化，但与此同时，与这种纳米级功能协同作用的生物活性化学增强这种作用的潜力尚未得到很大程度的考虑。本文介绍了对间质干细胞对沉积在多晶钛纳米结构表面的共形生物活性磷酸钙薄膜的反应的研究，具有直接诱导人骨髓间充质干细胞成骨分化的能力。溅射沉积的表面支持高水平的人类骨髓来源的间充质干细胞的粘附和增殖，这是通过DNA定量确定的。此外，还发现它们能够直接促进显着水平的成骨分化。具体来说，碱性磷酸酶活性，基因表达和关键成骨标记物的免疫细胞化学定位揭示了纳米结构的钛表面和生物活性磷酸钙涂层可以将分化方向导向成骨细胞系。此外，与在没有底层纳米结构的钛或磷酸钙涂层中观察到的情况相比，在钛的地形图中添加磷酸钙化学物质可诱导人骨髓间充质干细胞分化的增加。因此，此处提出的结果突出表明，将表面工程策略与定义的表面形貌与伴随的共形生物活性化学相结合，可以增强人类骨髓来源的间充质干细胞的直接成骨分化能力，这可以从表面工程策略中获得明显的收益。

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