...
首页> 外文期刊>Journal of Tissue Engineering >The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro
【24h】

The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro

机译:不同生物和生物合成伤口覆盖物对体外角质形成细胞生长,分层和分化的影响

获取原文
           

摘要

The purpose of this study was to compare, by means of in vitro cultivation technique, five marketed brands of wound covers used in the treatment of burns and other skin defects (Biobrane?, Suprathel?, Veloderm?, Xe-Derma?, and Xenoderm?) for their ability to stimulate the keratinocyte growth, stratification, and differentiation. In three independent experiments, human keratinocytes were grown on the tested covers in organotypic cultures by the 3T3 feeder layer technique. Vertical paraffin sections of the wound covers with keratinocytes were processed using hematoxylin–eosin staining and immunostaining for involucrin. Keratinocyte populations on the dressings were assessed for (1) number of keratinocyte strata (primary variable), (2) quantitative growth, (3) thickness of the keratinocyte layer, and (4) cell differentiation. The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata significantly (p?
机译:这项研究的目的是通过体外培养技术比较五个市场品牌的伤口覆盖物,用于治疗烧伤和其他皮肤缺陷(Biobrane?,Suprathel?,Veloderm?,Xe-Derma?和Xenoderm)。 ?)刺激角质形成细胞生长,分层和分化的能力。在三个独立的实验中,通过3T3饲养层技术,使人角质形成细胞在器官型培养物中的被测盖上生长。使用苏木精-伊红染色和对蛋清进行免疫染色处理带有角质形成细胞的伤口覆盖物的垂直石蜡切片。评估敷料上的角质形成细胞群的数量:(1)角质形成细胞层数(主要变量),(2)定量生长,(3)角质形成细胞层厚度和(4)细胞分化。 Xe-Derma伤口覆盖物为角质形成细胞的增殖和分层提供了最好的支持,除Xenoderm外,与所有研究产品相比,角质形成细胞的层数显着增加(p 0.05)。但是,与Xe-Derma相比,Xenoderm与其他敷料没有显着差异。体外研究结果表明,基于猪真皮基质的品牌对角质形成细胞的增殖和分层具有最强的作用。 Xe-Derma的独特位置可能与其成分有关,天然真皮纤维形成光滑的表面,类似于基底膜。此外,结果表明体外评估对上皮生长的影响可能会加速基于生物工程的新型伤口覆盖物的开发。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号