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首页> 外文期刊>Journal of Tissue Engineering >Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis
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Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis

机译：生长因子和超声辅助生物反应器对人间充质干细胞软骨形成的协同作用

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Ultrasound at 5.0?MHz was noted to be chondro-inductive, with improved SOX-9 gene and COL2A1 protein expression in constructs that allowed for cell-to-cell contact. To achieve tissue-engineered cartilage using macroporous scaffolds, it is hypothesized that a combination of ultrasound at 5.0?MHz and transforming growth factor-β3 induces human mesenchymal stem cell differentiation to chondrocytes. Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion. Our results suggest that in group 1 (no transforming growth factor-β3, no ultrasound), as anticipated, human mesenchymal stem cells were not efficiently differentiated into chondrocytes, judging by the lack of decrease in the level of miR-145 expression. Human mesenchymal stem cells differentiated into chondrocytes in group 2 (transforming growth factor-β3, no ultrasound) and group 3 (transforming growth factor-β3, ultrasound) with group 3 having a 2-fold lower miR-145 when compared to group 2 at day 7, indicating a higher conversion to chondrocytes. Transforming growth factor-β3–induced chondrogenesis with and without ultrasound stimulation for 14?days in the ultrasound-assisted bioreactor was compared and followed by additional culture in the absence of growth factors. The combination of growth factor and ultrasound stimulation (group 3) resulted in enhanced COL2A1, SOX-9, and ACAN protein expression when compared to growth factor alone (group 2). No COL10A1 protein expression was noted. Enhanced cell proliferation and glycosaminoglycan deposition was noted with the combination of growth factor and ultrasound stimulation. These results suggest that ultrasound at 5.0?MHz could be used to induce chondrogenic differentiation of mesenchymal stem cells for cartilage tissue engineering.
机译：在5.0？MHz的超声被认为是软骨诱导的，在允许细胞与细胞接触的构建体中，SOX-9基因和COL2A1蛋白表达得到改善。为了使用大孔支架实现组织工程化的软骨，假设在5.0？MHz的超声和转化生长因子-β3的组合下诱导人间充质干细胞向软骨细胞的分化。 miR-145的表达用作度量，定性评估人间充质干细胞转化的功效。我们的结果表明，如预期的那样，在第1组（无转化生长因子-β3，无超声）中，根据miR-145表达水平缺乏的下降来判断，人间充质干细胞不能有效地分化为软骨细胞。人间充质干细胞在第2组（转化生长因子-β3，无超声）和第3组（转化生长因子-β3，超声）中分化为软骨细胞，而第3组的miR-145降低了2倍，而第7天，表明向软骨细胞的转化更高。比较了在超声辅助的生物反应器中在有和没有超声刺激的情况下转化生长因子-β3诱导的软骨形成，持续了14天，然后在没有生长因子的情况下进行了额外的培养。与单独使用生长因子（第2组）相比，将生长因子和超声刺激（第3组）结合使用可增强COL2A1，SOX-9和ACAN蛋白的表达。没有注意到COL10A1蛋白表达。结合生长因子和超声刺激，注意到细胞增殖和糖胺聚糖沉积增强。这些结果表明，可以将5.0？MHz的超声用于诱导软骨组织工程的间充质干细胞的软骨分化。

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