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首页> 外文期刊>Journal of Xenobiotics >Bioremediation of refinery wastewater using immobilised Burkholderia cepacia and Corynebacterium sp and their transconjugants
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Bioremediation of refinery wastewater using immobilised Burkholderia cepacia and Corynebacterium sp and their transconjugants

机译：固定化洋葱伯克霍尔德菌和棒状杆菌及其转导结合物对炼油废水的生物修复

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When oil spill occurs, it poses serious toxic hazards to all forms of life. Mixed culture of Burkholderia cepacia and Corynebacterium sp isolated from refinery sludge using selective enrichment technique was used for bioremediation of refinery wastewater in a laboratoryscale bioreactor. Physicochemical parameters of both raw and treated water were as determined and compared with Federal Environ - mental Protection Agency (FEPA-limit, Abuja, Nigeria) to asses the efficiency of the bioremediation process. Each of the bacterium was screened for the presence of plasmid DNA and for the involvement or otherwise of plasmid in the bioremediation of wastewater. The immobilised cells showed percentage decrease in chemical oxygen demand (97%), biochemical oxygen demand (94%), phenol (98%), total petroleum hydrocarbon (79%), oil and grease (90%) of the refinery waste water after 20 days of treatment while their transconjugants showed the multiplicative effect by achieving the same percentage after 10 days of treatment. Therefore, the findings revealed that bioaugmentation of wastewater using transmissible catabolic plasmid will enhance efficiency of the bioremediation by spreading the plasmid among indigenous microbial community either through horizontal gene transfer or transformation.
机译：发生漏油事故时，会对所有形式的生命造成严重的毒性危害。使用选择性富集技术从炼油厂污泥中分离的洋葱伯克霍尔德菌和棒状杆菌的混合培养物用于实验室规模的生物反应器中的炼油废水的生物修复。确定了原水和处理后水的理化参数，并将其与联邦环境保护局（FEPA-limit，尼日利亚阿布贾）进行比较，以评估生物修复过程的效率。筛选每种细菌中质粒DNA的存在以及质粒是否参与废水的生物修复。固定化单元显示，精炼废水的化学需氧量（97％），生化需氧量（94％），苯酚（98％），总石油烃（79％），油脂（90％）减少了百分比治疗20天，而其转导结合剂则在治疗10天后达到相同的百分比，从而显示出增效作用。因此，研究结果表明，使用可传播的分解代谢质粒对废水进行生物强化可通过将质粒通过水平基因转移或转化传播到土著微生物群落中来提高生物修复效率。

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