• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Young Pharmacists >Inhibition of HIV-1 Reverse Transcriptase of Selected Indonesia Medicinal Plants and Isolation of the Inhibitor from Erythrina variegata L. Leaves
【24h】

Inhibition of HIV-1 Reverse Transcriptase of Selected Indonesia Medicinal Plants and Isolation of the Inhibitor from Erythrina variegata L. Leaves

机译：选定的印度尼西亚药用植物对HIV-1逆转录酶的抑制作用和从Erythrina variegata L.叶片中的抑制剂的分离

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: This research was conducted to screen inhibition of HIV-1 reverse transcriptase activity of selected Indonesia medicinal plants and to isolate HIV-1 reverse transcriptase inhibitor from Erythrina variegata leaves. Method: Screening inhibition of HIV-1 RT activity of selected Indonesia medicinal plants and isolated compounds were performed using HIV-1 RT colorimetric assay. The isolation of HIV-1 reverse transcriptase inhibitor was conducted using chromatography technique. The isolated compound was determined based on the data of UV, IR spectrophotometry, MS, 1D and 2D NMR spectroscopy. Results: Beside that, in vitro study of the leaves methanolic extract exhibited inhibition against HIV-1 RT activity with percent inhibition of 97.64% at concentration of 5 mg/mL. Ethyl acetate fraction from the extract showed the strongest HIV-1 RT inbitory activity with IC50 of 429.28 μg/mL. Isolation the HIV-1 RT inhibitor from the fraction give compound 1. Conclusion: Erythryna variegata leave extract exhibited potent inhibition on HIV-1 RT activity. The isolated compound from the leaves was determined as apigenin-7-O-β-D-glucopyranoside and demonstrated HIV-1 RT inhibitory activity with the IC50 value of 100.59 μg/mL.
机译：目的：本研究旨在筛选抑制的印度尼西亚药用植物对HIV-1逆转录酶活性的抑制作用，并从斑叶刺桐中分离HIV-1逆转录酶抑制剂。方法：使用HIV-1 RT比色法筛选所选印度尼西亚药用植物对HIV-1 RT活性的抑制作用，并分离出化合物。 HIV-1逆转录酶抑制剂的分离采用色谱技术进行。根据UV，IR分光光度法，MS，1D和2D NMR光谱数据确定分离的化合物。结果：此外，叶片甲醇提取物的体外研究显示出对HIV-1 RT活性的抑制作用，在5 mg / mL的浓度下抑制百分率为97.64％。提取物中的乙酸乙酯组分显示出最强的HIV-1 RT插入活性，IC 50 为429.28μg/ mL。从级分中分离出HIV-1 RT抑制剂，得到化合物1。结论：紫叶提取物表现出对HIV-1 RT活性的有效抑制作用。从叶中分离出的化合物被确定为芹菜素-7-O-β-D-吡喃葡萄糖苷，并具有HIV-1 RT抑制活性，IC <50>值为100.59μg/ mL。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号