• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Young Pharmacists >UV-Vis Spectroscopy to Enable Determination of the Dissolution Behavior of Solid Dispersions Containing Curcumin and Piperine
【24h】

UV-Vis Spectroscopy to Enable Determination of the Dissolution Behavior of Solid Dispersions Containing Curcumin and Piperine

机译：紫外-可见光谱法能够测定含有姜黄素和胡椒碱的固体分散体的溶解行为

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: Curcumin and piperine exibit poor aqueous solubility and dissolution. Solid dispersion technology is a promising way to improve dissolution of lipophilic compounds. Therefore, to investigate the dissolution behavior, an accurate determination of curcumin and piperine concentrations in a sample containing 0.5 wt-% SLS in sodium phosphate buffer as the dissolution medium was required. The goal of research was to investigate whether UV-Vis spectroscopy can be used to accurately determine curcumin and piperine concentrations in aqueous solutions. Method: Stock solutions (1 mg/mL) of curcumin and piperine were prepared in methanol. A series of solutions for calibration were prepared by mixing the stock solutions in various ratios after which they were diluted with dissolution medium (0.5%-wt sodium lauryl sulfate in 20 mM phosphate buffer; pH 6.0). Solutions of curcumin (2μg/mL) and piperine (1 μg/mL were subjected to overlay scan in a UV-VIS spectrophotometer in each λsubmax/sub of 430 and 335.5 nm for curcumin and piperine, respectively. The method was validated according ICH requirements, such as specificity, linearity, accuracy, precision, limit of detection and limit of quantification. Results: At a concentration range of 0.1 to 5μg/mL, calibration curves of curcumin and piperine showed linearity with Rsup2/sup = 0.9980 and 0.9982, respectively. Precision and accuracy was confirmed by AOAC. Furthermore, LOD of both compounds was 0.23 μg/mL and LOQ of curcumin and piperine was 0.72 and 0.69μg/mL, respectively. Conclusion: UV-Vis spectroscopy can be used to accurately determine curcumin and piperine concentrations in dissolution samples obtained during screening the dissolution behavior of solid dispersions containing these compounds.
机译：目的：姜黄素和胡椒碱的水溶性和溶解性差。固体分散技术是改善亲脂性化合物溶解的一种有前途的方法。因此，为了研究溶解行为，需要精确测定在磷酸钠缓冲液中含有0.5 wt％SLS作为溶解介质的样品中姜黄素和胡椒碱的浓度。研究的目的是研究紫外可见光谱是否可用于准确测定水溶液中的姜黄素和胡椒碱浓度。方法：在甲醇中制备姜黄素和胡椒碱的储备溶液（1 mg / mL）。通过将储备溶液以各种比例混合来制备一系列用于校准的溶液，然后将它们用溶解介质（在20 mM磷酸盐缓冲液中的0.5％-wt月桂基硫酸钠; pH 6.0）稀释。姜黄素（2μg/ mL）和胡椒碱（1μg/ mL）的溶液在紫外可见分光光度计中分别以姜黄素和胡椒碱的λmax分别为430和335.5 nm进行覆盖扫描。结果：在0.1-5μg/ mL的浓度范围内，姜黄素和胡椒碱的校正曲线呈线性关系，R 2 分别为0.9980和0.9982，AOAC证实了精密度和准确性，而且两种化合物的LOD分别为0.23μg/ mL，姜黄素和胡椒碱的LOQ分别为0.72和0.69μg/ mL。紫外可见光谱法可用于准确测定在筛选含有这些化合物的固体分散体的溶解行为时获得的溶解样品中的姜黄素和胡椒碱浓度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号