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首页> 外文期刊>Journal of Yeast and Fungal Research >Isolation and characterization of fungi from a hot-spring on the shores of Lake Bogoria, Kenya
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Isolation and characterization of fungi from a hot-spring on the shores of Lake Bogoria, Kenya

机译：肯尼亚柏哥利亚湖岸边温泉中真菌的分离与鉴定

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Hot springs are aquatic environments with high temperatures. They harbor diverse groups of micro-organisms like fungi that have developed mechanisms to thrive at wide temperature ranges, according to their optimal growth requirements. Fungi are a large group of eukaryotic organisms with worldwide distribution, inhabiting a diverse range of extreme habitats from deserts to hyper saline environments. The fungal diversity of the hot spring along the shores of Lake Bogoria was investigated using a culture-dependent approach. Microbial mats and wet sediments were collected from three sampling points along the hot spring while water samples were gotten from the mouth of the hot spring on the shores of the lake. Physicochemical characteristics were recorded at the study sites. Samples from the hot spring were isolated on four different media at pH 10 and at a temperature of 30°C. A total of seventeen fungal isolates were identified using morphological, physiological and molecular characters. Furthermore, the isolates were screened for production of extracellular enzymes. All the fungal isolates variedly grew at pH ranging from 5 to 10, temperature range of 25 to 35°C and sodium chloride range of 5 to 30%. Based on experimental analysis, isolate B61 exhibited significant growth in the four parameters tested. Also all the seventeen isolates produced different extracellular enzymes such as amylases, lipases, proteases and esterases. Analysis of partial sequences using Blastn showed that about 11.7 and 29.1% of the isolates were affiliated with members belonging to the genera Penicillium and Aspergillus, respectively. A total of 12% of the isolates belonged to the genera Alternaria and Fusarium while another 41% of the isolates clustered closely with uncultured fungus. Fungal endophyte comprised 6%. Although the culture dependent method did not reveal the true diversity of the fungal community, the isolates recovered were a representation of thermotolerant, alkalitolerant and halotolerant microorganisms found in soda lake environments. The isolates have the potential to produce useful enzymes for biotechnological applications.
机译：温泉是高温的水生环境。它们包含不同种类的微生物，例如真菌，根据它们的最佳生长要求，它们已经开发出在较宽温度范围内壮成长的机制。真菌是一大类真核生物，分布在世界各地，栖息于从沙漠到高盐分环境的各种极端栖息地。博格利亚湖沿岸温泉的真菌多样性是采用一种依赖于文化的方法进行调查的。从沿温泉的三个采样点收集了微生物垫和湿的沉积物，而从湖岸的温泉口采集了水样。在研究地点记录了理化特性。将来自温泉的样品在pH 10和30°C的四种不同介质上分离。使用形态，生理和分子特征鉴定出总共十七种真菌分离物。此外，筛选分离物以产生细胞外酶。所有真菌分离物在5至10的pH，25至35°C的温度范围和5至30％的氯化钠范围内变化生长。基于实验分析，分离株B61在四个测试参数中均显示出显着增长。同样，所有十七种分离物均产生不同的细胞外酶，例如淀粉酶，脂肪酶，蛋白酶和酯酶。使用Blastn进行的部分序列分析表明，分离株中约有11.7和29.1％分别与属于青霉属和曲霉属的成员有关。共有12％的分离物属于链格孢属和镰刀菌属，而另外41％的分离物则与未培养的真菌紧密结合。真菌内生菌占6％。尽管依赖培养物的方法并不能揭示真菌群落的真正多样性，但回收的分离株代表了在苏打湖环境中发现的耐热，耐碱和耐卤代微生物。分离物具有产生用于生物技术应用的有用酶的潜力。

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