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首页> 外文期刊>Journal of Young Pharmacists >Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice
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Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice

机译：芦笋根提取物和异芥子碱对电离辐射致瑞士白化小鼠的致死性和毒性的保护作用。

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Objective: We aimed to evaluate anticlastogenic and radioprotective potential of Asparagus racemosus root extract (ARE) and Isoprinosine (IPR) against electron beam radiation (EBR) induced clastogenicity and toxicity in Swiss albino mice. Methodology: In the pre-radiation study, the experimental animals were orally administered ARE - 200mg and IPR - 400mg/ kg b.wt once daily for 15 consecutive days. The animals exposed to sublethal dose (6Gy) of whole body EBR. Chromosomal aberration analysis and micronucleus assay were carried out in the bone marrow cells of the experimental animals. The various types of aberrations were scored and the micronuclei in Polychromatic Erythrocytes (PCE) and Nomochromatic Erythrocytes (NCE) were recorded. Assessment of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was performed using mouse GM-CSF Picokine ELISA kit. Non-specific Alpha - esterase activity was determined by simultaneous azo dye coupling method. Dose Reduction Factor (DRF) was calculated to determine the protective role of ARE and IPR against EBR. Result: Treatment of mice with ARE-200 mg/kg b.wt and IPR-400mg/kg b.wt decreased the percentage of the total aberration compared to the irradiated group; significantly reduced (P0.05) the frequency of Mn PCE and Mn NCE when compared with irradiation alone groups. Irradiation reduced the level of GM-CSF in the splenocytes which was enhanced by the pre-treatment with ARE and IPR. There was a significant increase in the number of alpha-esterase positive cells in the pre-treatment group compared to radiation control. Increase in survival percentage was observed in the pre-treated mice when compared to radiation alone group. The DRF value of 1.11 and 1.04 was observed respectively. Conclusion: The present study suggests that the antioxidant potential of ARE and IPR could be of extreme significance in offering radioprotection and may be useful in combating various free-radical and reactive oxygen species - mediated human pathological conditions.
机译：目的：我们旨在评估瑞士白化芦笋中芦笋根提取物（ARE）和异麦芽碱（IPR）对电子束辐射（EBR）诱导的杀伤力和毒性的抗肿瘤和放射防护作用。方法：在放疗前的研究中，连续15天每天一次给动物口服ARE-200mg和IPR-400mg / kg b.wt口服。动物暴露于致死剂量（6Gy）的全身EBR中。在实验动物的骨髓细胞中进行了染色体畸变分析和微核分析。对各种类型的像差进行评分，并记录多色红细胞（PCE）和非色红细胞（NCE）中的微核。使用小鼠GM-CSF Picokine ELISA试剂盒评估粒细胞巨噬细胞集落刺激因子（GM-CSF）。通过同时偶氮染料偶联法测定非特异性α-酯酶活性。计算剂量减少因子（DRF），以确定ARE和IPR对EBR的保护作用。结果：与照射组相比，ARE-200 mg / kg b.wt和IPR-400mg / kg b.wt的小鼠治疗降低了总像差百分比;与单独照射组相比，Mn PCE和Mn NCE的频率显着降低（P <0.05）。辐照降低了脾细胞中GM-CSF的水平，这通过ARE和IPR的预处理得以增强。与辐射对照相比，预处理组中的α-酯酶阳性细胞数量显着增加。与单独放疗组相比，在预处理小鼠中观察到存活率增加。 DRF值分别为1.11和1.04。结论：本研究表明，ARE和IPR的抗氧化潜力在提供放射防护方面可能具有极其重要的意义，并且可能在对抗各种自由基和活性氧物种介导的人类病理状况中有用。

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