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首页> 外文期刊>Journal of Translational Medicine >Semi-allogeneic vaccine for T-cell lymphoma
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Semi-allogeneic vaccine for T-cell lymphoma

机译：T细胞淋巴瘤半同种异体疫苗

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Background Experimental results from studies with inbred mice and their syngeneic tumors indicated that the inoculation of semi-allogeneic cell hybrids (derived from the fusion between syngeneic tumor cells and an allogeneic cell line) protects the animal host from a subsequent lethal challenge with unmodified syngeneic tumor cells. Methods Semi-allogeneic somatic cell hybrids were generated by the fusion of EL-4 T lymphoma cells (H-2b) and BALB/c-derived renal adenocarcinoma RAG cells (H-2d). Cell hybrids were injected intra-peritoneally (i.p.) in C57BL/6 mice (H-2b) before challenging the mice with a tumorigenic dose of EL-4 cells. Results Semi-allogeneic tumor cell hybrids could not form a tumor in the animal host because they expressed allogeneic determinants (H-2d) and were rejected as a transplant. However, they conferred protection against a tumorigenic challenge of EL-4 cells compared to control mice that were mock-vaccinated with i.p.-injected phosphate-buffered saline (PBS) and in which EL-4 lymphomas grew rapidly to a large size in the peritoneal cavity. Screening of spleen-derived RNA by means of focused microarray technology revealed up-regulation of genes involved in the Th-1-type immune response and in the activation of dendritic antigen-presenting cells (APC). Conclusion The results of our studies are entirely consistent with the concept that CD80- and CD86-expressing APC play a central role in mediating the immune protection induced by semi-allogeneic vaccines by activating a Th-1 response and instructing T cells responsible for killing autologous tumor cells.
机译：背景近交小鼠及其同系肿瘤研究的实验结果表明，接种半同代异体细胞杂种（源自同系肿瘤细胞和同种异体细胞系之间的融合）可保护动物宿主免受随后未经修饰的同系肿瘤的致命攻击细胞。方法通过EL-4 T淋巴瘤细胞（H-2 b ）和BALB / c来源的肾腺癌RAG细胞（H-2 d）融合产生半异体体细胞杂种）。在向C57BL / 6小鼠（H-2 b ）腹膜内（i.p.）注射细胞杂种，然后用致瘤剂量的EL-4细胞攻击小鼠。结果半同种异体肿瘤细胞杂种不能在动物宿主中形成肿瘤，因为它们表达了同种异体决定簇（H-2 d ）并被拒绝移植。然而，与用ip注射的磷酸盐缓冲盐水（PBS）模拟接种的对照小鼠相比，它们赋予了针对EL-4细胞致瘤性攻击的保护作用，在对照小鼠中，EL-4淋巴瘤在腹膜中迅速生长至较大尺寸腔。借助聚焦微阵列技术筛选脾源性RNA，揭示了Th-1型免疫应答和树突状抗原呈递细胞（APC）激活相关基因的上调。结论我们的研究结果与CD80和CD86表达的APC通过激活Th-1应答并指导T细胞负责自体杀伤的介导在半同基因疫苗诱导的免疫保护中起着核心作用的概念完全一致。肿瘤细胞。

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