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首页> 外文期刊>Jundishapur Journal of Microbiology >In vitro Evaluation of the Effects of Fluconazole and Nano-Fluconazole on Aspergillus flavus and A. fumigatus Isolates
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In vitro Evaluation of the Effects of Fluconazole and Nano-Fluconazole on Aspergillus flavus and A. fumigatus Isolates

机译：氟康唑和纳米氟康唑对黄曲霉和烟曲霉分离物影响的体外评价

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Increasing azole resistance among Aspergillus strains was observed over the last decade. For this reason, viable alternatives for the current medicines are required.The current study aimed at producing fluconazole-loaded liposomal nanoparticles and comparing in vitro antifungal activity of fluconazole and nano-fluconazole on Aspergillus flavus and A. fumigatus species isolated from patients and poultry.Fifty isolates of A. flavus and A. fumigatus were collected from visceral and superficial fungal lesions of humans as well as pulmonary fungal infection of poultry. Liposomal nanoparticles were prepared by thin-film hydration method, using soybean lecithin, cholesterol, and fluconazole in a ratio of 10:1:1. The nanoparticles were analyzed in terms of size, zeta potential, and morphology. Antifungal susceptibility testing was carried out to examine and evaluate the minimum inhibitory concentration (MIC) of fluconazole and nano-fluconazole against Aspergillus species by the standard method of broth microdilution as described in CLSI (the clinical and laboratory standards institute) -M38A2.The particle size of liposomes containing fluconazole was 88.9 ?± 12.1 nm and its zeta potential was -20.12 ?± 1.88 mv. Nano-liposomes containing fluconazole significantly reduced the MIC against A. flavus (P = 0.0005) and A. fumigatus (P & 0.0001).The results of the current in vitro study showed that nano-fluconazole has better antifungal effects than the common form of drug on A. flavus and A. fumigatus species. The current study showed the antifungal activity of nano-drugs.
机译：在过去的十年中，观察到曲霉菌株之间的唑抗性增加。因此，目前的药物是可行的替代品。本研究旨在生产载有氟康唑的脂质体纳米颗粒，并比较氟康唑和纳米氟康唑对从患者和家禽分离出的黄曲霉和烟曲霉的体外抗真菌活性。从人的内脏和浅表真菌病灶以及家禽的肺部真菌感染中收集了50株黄曲霉和烟曲霉。通过薄膜水化法，使用大豆卵磷脂，胆固醇和氟康唑以10：1：1的比例制备脂质体纳米颗粒。根据尺寸，ζ电势和形态分析了纳米颗粒。通过CLSI（临床和实验室标准协会）-M38A2中所述的标准肉汤微稀释方法，进行了抗真菌药敏试验，以检查和评估氟康唑和纳米氟康唑对曲霉菌的最低抑菌浓度（MIC）。含有氟康唑的脂质体的大小为88.9±12.1nm，ζ电位为-20.12±1.88mv。含有氟康唑的纳米脂质体显着降低了针对黄曲霉（P = 0.0005）和烟曲霉（P <0.0001）的MIC。目前的体外研究结果表明，纳米氟康唑具有比普通形式更好的抗真菌作用药物对黄曲霉和烟曲霉种类的影响。当前的研究显示了纳米药物的抗真菌活性。

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