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首页> 外文期刊>Jundishapur Journal of Microbiology >Extended-Spectrum Beta-Lactamases in Cystic Fibrosis Isolates of Klebsiella pneumoniae
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Extended-Spectrum Beta-Lactamases in Cystic Fibrosis Isolates of Klebsiella pneumoniae

机译：肺炎克雷伯菌的囊性纤维化分离物中的广谱β-内酰胺酶

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Background: The emergence and spread of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are recognized as a major clinical problem and a public health challenge. Enterobacteria are transient colonizers of lungs affected by cystic fibrosis (CF). Klebsiella pneumoniae has been shown to cause chronic obstructive pulmonary infections. Objectives: We aimed to determine the antibiotic resistance profile and ESBL production in K. pneumoniae isolates from Iranian patients with CF. Methods: Sixteen K. pneumoniae strains were isolated from the sputum samples of 98 pediatric CF patients at Mofid children’s hospital in Tehran, Iran. Antibiotic susceptibility was determined via disc diffusion, and ESBL phenotype was detected by double disc synergy test (DDST). β-lactamase genes, including TEM, SHV, CTX-M, and OXA genes, were detected using polymerase chain reaction (PCR) and confirmed by sequencing the PCR products. Finally, genetic fingerprints of the isolates were determined via random amplified polymorphic DNA (RAPD)-PCR method. Results: Ten isolates had ESBL phenotypes, all of which contained non-ESBL blaTEM-1 genes. Seven isolates harbored blaCTX-M-15 genes (ESBL genes), five of which also showed non-ESBL blaSHV-11, blaTEM-1, and blaOXA-1 genes (1 isolate with blaTEM-1 and 1 isolate with blaTEM-1 plus blaOXA-1 gene). However, PER-1 and VEB-1 β-lactamase genes were not detected. Genetic fingerprinting profiles showed heterogeneity among K. pneumoniae isolates. Conclusions: This study highlights not only the presence of multiple β-lactamases, but also the carriage of ESBL blaCTX-M-15 gene among K. pneumoniae isolates from CF patients
机译：背景：产生广谱β-内酰胺酶（ESBL）的肠杆菌科细菌的出现和扩散被认为是主要的临床问题和公共卫生挑战。肠杆菌是受囊性纤维化（CF）影响的肺的短暂定居者。肺炎克雷伯菌已被证明可引起慢性阻塞性肺部感染。目的：我们旨在确定伊朗CF患者肺炎克雷伯菌中的耐药性和ESBL产生情况。方法：在伊朗德黑兰的Mofid儿童医院从98例小儿CF患者的痰液样本中分离出16株肺炎克雷伯菌。通过盘扩散确定抗生素敏感性，并通过双盘协同试验（DDST）检测ESBL表型。使用聚合酶链反应（PCR）检测β-内酰胺酶基因，包括TEM，SHV，CTX-M和OXA基因，并通过对PCR产物进行测序来确认。最后，通过随机扩增多态性DNA（RAPD）-PCR方法确定了分离株的遗传指纹。结果：十株分离株具有ESBL表型，均含有非ESBL blaTEM-1基因。七个分离株带有blaCTX-M-15基因（ESBL基因），其中五个还显示了非ESBL blaSHV-11，blaTEM-1和blaOXA-1基因（1个分离株为blaTEM-1，1个分离株为blaTEM-1 plus blaOXA-1基因）。然而，未检测到PER-1和VEB-1β-内酰胺酶基因。遗传指纹图谱显示肺炎克雷伯菌分离株之间的异质性。结论：这项研究不仅强调了多种β-内酰胺酶的存在，而且强调了CF患者肺炎克雷伯菌中ESBL blaCTX-M-15基因的携带。

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