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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China
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Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

机译：上海市郊区水痘带状疱疹病毒分离株的基因型分析

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摘要

To determine the predominant genotype of the varicella-zoster virus (VZV) in suburban Shanghai Municipal Province, specimens were collected from the lesions of 95 outpatients clinically diagnosed with varicella or herpes zoster. Of these, 69 patients (72.6?%) were positive for VZV DNA. The 69 isolates were all genotyped as the genotype J1/clade 2. Based on sequencing of the 447?bp sequence in ORF22, 66 isolates were identified as genotype J/clade 2 strains and three were identified as type M2/clade 4 strains. To confirm the classification of these three strains, we determined the presence of 27 single-nucleotide polymorphisms (SNPs) and found that isolates 1270/1450 shared seven SNPs that differed from those of clade 2, in which three SNPs were unique to clade 3 and another three were unique to clade 4. Isolate 1456 had two markers of clade 4 that differed from clade 2. The phylogenetic tree showed that our isolates clustered primarily with clade 2 and that the three M2/J1 strains clustered between clades 2 and 4. It is likely that isolates 1270/1450/1446 may represent a new subclade of either clade 2 or 4, or some recombinant events. In addition, our isolates were WT strains. We also observed significant inter-strain variations.
机译：为了确定上海郊区的水痘带状疱疹病毒（VZV）的主要基因型，从临床诊断为水痘或带状疱疹的95名门诊患者的病灶中收集标本。其中69例（72.6％）的VZV DNA阳性。 69个分离株均被定型为基因型J1 / clade2。基于ORF22中447？bp序列的测序，鉴定出66个分离株为基因型J / clade 2菌株，三个被鉴定为M2 / clade 4菌株。为了确认这三个菌株的分类，我们确定了27个单核苷酸多态性（SNP）的存在，并发现分离株1270/1450共有7个不同于进化枝2的SNP，其中三个SNP在进化枝3和3中是唯一的。进化枝4特有另外三个。分离株1456具有不同于进化枝2的进化枝4的两个标记。系统进化树表明，我们的分离株主要与进化枝2聚在一起，并且三个M2 / J1菌株聚在进化枝2和4之间。分离株1270/1450/1446可能代表进化枝2或4的新子亚群或某些重组事件。另外，我们的分离株是野生型菌株。我们还观察到了显着的株间差异。

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