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首页> 外文期刊>BioMed research international >Generation of Biotechnology-DerivedFlavobacterium columnareGhosts by PhiX174 GeneE-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp
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Generation of Biotechnology-DerivedFlavobacterium columnareGhosts by PhiX174 GeneE-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp

机译：通过PhiX174 GeneE介导的失活产生生物技术衍生的黄杆菌GhostesGhosts以及抗草鱼感染的疫苗候选疫苗的潜力

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Flavobacterium columnareis a bacterial pathogen causing high mortality rates for many freshwater fish species. Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease. Here, in order to produce bacterial ghost vaccine, a specificFlavobacteriumlysis plasmid pBV-E-cat was constructed by cloning PhiX174 lysis geneEand thecatgene with the promoter ofF. columnareinto the prokaryotic expression vector pBV220. The plasmid was successfully electroporated into the strainF. columnareG4cpN22 after curing of its endogenous plasmid.F. columnareG4cpN22 ghosts (FCGs) were generated for the first time by geneE-mediated lysis, and the vaccine potential of FCG was investigated in grass carp (Ctenopharyngodon idellus) by intraperitoneal route. Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS. Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%). These results showed that FCG could confer immune protection againstF. columnareinfection. As a nonliving whole cell envelope preparation, FCG may provide an ideal alternative to pathogen-based vaccines against columnaris in aquaculture.
机译：柱状黄杆菌是引起许多淡水鱼物种高死亡率的细菌病原体。用安全有效的疫苗接种鱼是预防和控制鱼病的一种潜在方法。在此，为了生产细菌幽灵疫苗，通过克隆PhiX174裂解基因E和具有F启动子的猫基因，构建了特定的黄杆菌裂解质粒pBV-E-cat。 columnarein原核表达载体pBV220。质粒成功地电穿孔到菌株F中。固化内源质粒后，将其列为G4cpN22。通过geneE介导的裂解首次产生了columnareG4cpN22幽灵（FCG），并通过腹膜内途径在草鱼（Ctenopharyngodon idellus）中研究了FCG的疫苗潜力。与FKC或PBS免疫的鱼相比，FCG免疫的鱼显示出更高的血清凝集滴度和杀菌活性。最重要的是，在用亲本菌株G4攻击后，FCG组（70.9％）的鱼类相对存活率（RPS）显着高于FKC组（41.9％）。这些结果表明，FCG可以赋予针对F的免疫保护。柱状感染。作为无生命的全细胞包膜制剂，FCG可以为水产养殖中针对柱状杆菌的基于病原体的疫苗提供理想的替代品。

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