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首页> 外文期刊>BioMed research international >Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester:In VitroAssays
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Fibrinolytic Activity and Dose-Dependent Effect of Incubating Human Blood Clots in Caffeic Acid Phenethyl Ester:In VitroAssays

机译：在咖啡酸苯乙基酯中孵育人血凝块的纤溶活性和剂量依赖性效应：体外测定

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Background. Caffeic acid phenethyl ester (CAPE) has been reported to possess time-dependent fibrinolytic activity byin vitroassay. This study is aimed at investigating fibrinolytic dose-dependent activity of CAPE usingin vitroassays.Methods. Standardized human whole blood (WB) clots were incubated in either blank controls or different concentrations of CAPE (3.75, 7.50, 15.00, 22.50, and 30.00 mM). After 3 hours, D-dimer (DD) levels and WB clot weights were measured for each concentration. Thromboelastography (TEG) parameters were recorded following CAPE incubation, and fibrin morphology was examined under a confocal microscope.Results. Overall, mean DD (μg/mL) levels were significantly different across samples incubated with different CAPE concentrations, and the median pre- and postincubation WB clot weights (grams) were significantly decreased for each CAPE concentration. Fibrin removal was observed microscopically and indicated dose-dependent effects. Based on the TEG test, the Ly30 fibrinolytic parameter was significantly different between samples incubated with two different CAPE concentrations (15.0 and 22.50 mM). The 50% effective dose (ED50) of CAPE (based on DD) was 1.99 mg/mL.Conclusions. This study suggests that CAPE possesses fibrinolytic activity followingin vitroincubation and that it has dose-dependent activities. Therefore, further investigation into CAPE as a potential alternative thrombolytic agent should be conducted.
机译：背景。据报道，通过体外测定，咖啡酸苯乙酯（CAPE）具有随时间变化的纤溶活性。本研究旨在利用体外测定方法研究CAPE的纤溶剂量依赖性活性。在空白对照或不同浓度的CAPE（3.75、7.50、15.00、22.50和30.00mM）中孵育标准的人类全血（WB）血块。 3小时后，测量每种浓度的D-二聚体（DD）水平和WB凝块重量。 CAPE孵育后记录血栓弹力图（TEG）参数，并在共聚焦显微镜下检查纤维蛋白形态。总体而言，在不同CAPE浓度下孵育的样品之间，平均DD（μg/ mL）水平存在显着差异，并且每种CAPE浓度均会降低孵育前和孵育后WB凝块的重量中位数（克）。在显微镜下观察到纤维蛋白的去除并显示出剂量依赖性作用。根据TEG测试，在用两种不同的CAPE浓度（15.0和22.50μmM）孵育的样品之间，Ly30的纤溶参数明显不同。 CAPE的50％有效剂量（ED50）（基于DD）为1.99μg/ mL。这项研究表明，CAPE在体外孵育后具有纤溶活性，并且具有剂量依赖性活性。因此，应该对CAPE作为一种潜在的溶栓剂进行进一步的研究。

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