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首页> 外文期刊>BMC Genomics >Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli
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Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli

机译：小型非编码RNA GcvB是大肠杆菌中耐酸性的新型调节剂

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Background The low pH environment of the human stomach is lethal for most microorganisms; but not Escherichia coli, which can tolerate extreme acid stress. Acid resistance in E. coli is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA). Results In this study, we individually deleted seventy-nine sncRNA genes from the E. coli K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in E. coli. We demonstrate that GcvB enhances the ability of E. coli to survive low pH by upregulating the levels of the alternate sigma factor RpoS. Conclusion GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli.
机译：背景技术人胃的低pH环境对大多数微生物具有致命性。但不能耐受极端酸胁迫的大肠杆菌。大肠杆菌中的耐酸性受到众多调节剂的分级控制，其中包括小的非编码RNA（sncRNA）。结果在这项研究中，我们从大肠杆菌K12-MG1655染色体中单独删除了79个sncRNA基因，并建立了一个单sncRNA基因敲除文库。通过系统地筛选sncRNA突变体文库，我们显示sncRNA GcvB是大肠杆菌中耐酸性的新型调节剂。我们证明，GcvB通过上调备用sigma因子RpoS的水平来增强大肠杆菌在低pH条件下生存的能力。结论GcvB通过影响RpoS的表达正调控酸抵抗。这些数据使我们对参与调控大肠杆菌抗酸性的sncRNA调控网络有了更深入的了解。

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