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首页> 外文期刊>BMC Genomics >Identification of genes expressed in the sex pheromone gland of the black cutworm Agrotis ipsilon with putative roles in sex pheromone biosynthesis and transport
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Identification of genes expressed in the sex pheromone gland of the black cutworm Agrotis ipsilon with putative roles in sex pheromone biosynthesis and transport

机译：鉴定在黑鳞甲虫（Agrotis ipsilon）性信息素腺中表达的基因，该基因在性信息素的生物合成和运输中具有假定作用

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Background One of the challenges in insect chemical ecology is to understand how insect pheromones are synthesised, detected and degraded. Genome wide survey by comparative sequencing and gene specific expression profiling provide rich resources for this challenge. A. ipsilon is a destructive pest of many crops and further characterization of the genes involved in pheromone biosynthesis and transport could offer potential targets for disruption of their chemical communication and for crop protection. Results Here we report 454 next-generation sequencing of the A. ipsilon pheromone gland transcriptome, identification and expression profiling of genes putatively involved in pheromone production, transport and degradation. A total of 23473 unigenes were obtained from the transcriptome analysis, 86% of which were A. ipsilon specific. 42 transcripts encoded enzymes putatively involved in pheromone biosynthesis, of which 15 were specifically, or mainly, expressed in the pheromone glands at 5 to 120-fold higher levels than in the body. Two transcripts encoding for a fatty acid synthase and a desaturase were highly abundant in the transcriptome and expressed more than 40-fold higher in the glands than in the body. The transcripts encoding for 2 acetyl-CoA carboxylases, 1 fatty acid synthase, 2 desaturases, 3 acyl-CoA reductases, 2 alcohol oxidases, 2 aldehyde reductases and 3 acetyltransferases were expressed at a significantly higher level in the pheromone glands than in the body. 17 esterase transcripts were not gland-specific and 7 of these were expressed highly in the antennae. Seven transcripts encoding odorant binding proteins (OBPs) and 8 encoding chemosensory proteins (CSPs) were identified. Two CSP transcripts (AipsCSP2, AipsCSP8) were highly abundant in the pheromone gland transcriptome and this was confirmed by qRT-PCR. One OBP (AipsOBP6) were pheromone gland-enriched and three OBPs (AipsOBP1, AipsOBP2 and AipsOBP4) were antennal-enriched. Based on these studies we proposed possible A. ipsilon biosynthesis pathways for major and minor sex pheromone components. Conclusions Our study identified genes potentially involved in sex pheromone biosynthesis and transport in A. ipsilon. The identified genes are likely to play essential roles in sex pheromone production, transport and degradation and could serve as targets to interfere with pheromone release. The identification of highly expressed CSPs and OBPs in the pheromone gland suggests that they may play a role in the binding, transport and release of sex pheromones during sex pheromone production in A. ipsilon and other Lepidoptera insects.
机译：背景技术昆虫化学生态学中的挑战之一是了解昆虫信息素是如何合成，检测和降解的。通过比较测序和基因特异性表达谱进行的全基因组调查为应对这一挑战提供了丰富的资源。 A. ipsilon是许多农作物的破坏性害虫，对信息素生物合成和运输中涉及的基因的进一步表征可能为破坏其化学通讯和保护作物提供潜在目标。结果在这里，我们报告了A. ipsilon信息素腺转录组的454下一代测序，推定了涉及信息素产生，运输和降解的基因的鉴定和表达谱。通过转录组分析获得了总共23473个单基因，其中86％是A. ipsilon特异的。 42种转录本编码可能参与信息素生物合成的酶，其中15种在信息素腺中特异性或主要表达的水平比体内高5至120倍。编码脂肪酸合酶和去饱和酶的两个转录本在转录组中高度丰富，并且在腺体中的表达量比体内高40倍以上。编码2个乙酰基-CoA羧化酶，1个脂肪酸合酶，2个去饱和酶，3个酰基-CoA还原酶，2个醇氧化酶，2个醛还原酶和3个乙酰基转移酶的转录本在信息素腺中的表达水平明显高于体内。 17个酯酶转录本不是腺体特异性的，其中7个在触角中高表达。鉴定了七个编码气味结合蛋白（OBP）的转录本和八个编码化学感觉蛋白（CSP）的转录本。两个CSP转录本（AipsCSP2，AipsCSP8）在信息素腺转录组中高度丰富，这已通过qRT-PCR证实。一个OBP（AipsOBP6）富含信息素腺体，三个OBP（AipsOBP1，AipsOBP2和AipsOBP4）富含触角。基于这些研究，我们提出了主要和次要性信息素成分的可能的A. ipsilon生物合成途径。结论我们的研究确定了潜在的基因参与性信息素的生物合成和运输。鉴定出的基因可能在性信息素的产生，运输和降解中起重要作用，并且可以作为干扰信息素释放的靶标。在信息素腺中高表达的CSP和OBP的鉴定表明，它们可能在A. ipsilon和其他鳞翅目昆虫的性信息素产生过程中，在性信息素的结合，转运和释放中起作用。

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