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首页> 外文期刊>BMC Genomics >Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development
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Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development

机译：水果发育过程中两种橄榄基因型转录本的454焦磷酸测序比较

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Background Despite its primary economic importance, genomic information on olive tree is still lacking. 454 pyrosequencing was used to enrich the very few sequence data currently available for the Olea europaea species and to identify genes involved in expression of fruit quality traits. Results Fruits of Coratina, a widely cultivated variety characterized by a very high phenolic content, and Tendellone, an oleuropein-lacking natural variant, were used as starting material for monitoring the transcriptome. Four different cDNA libraries were sequenced, respectively at the beginning and at the end of drupe development. A total of 261,485 reads were obtained, for an output of about 58 Mb. Raw sequence data were processed using a four step pipeline procedure and data were stored in a relational database with a web interface. Conclusion Massively parallel sequencing of different fruit cDNA collections has provided large scale information about the structure and putative function of gene transcripts accumulated during fruit development. Comparative transcript profiling allowed the identification of differentially expressed genes with potential relevance in regulating the fruit metabolism and phenolic content during ripening.
机译：背景技术尽管具有重要的经济意义，但仍缺乏有关橄榄树的基因组信息。 454焦磷酸测序用于丰富目前对于油橄榄种的极少数序列数据，并鉴定涉及果实品质性状表达的基因。结果Coratina果实是一种广泛种植的品种，具有很高的酚含量，而Tendellone是一种缺少橄榄苦苷的天然变体，被用作监测转录组的起始材料。在核果发育的开始和结束时分别对四个不同的cDNA文库进行了测序。总共获得261,485次读取，输出约为58 Mb。使用四步流水线过程处理原始序列数据，并将数据存储在具有Web界面的关系数据库中。结论对不同果实cDNA集合进行大规模平行测序已提供了有关果实发育过程中积累的基因转录本的结构和假定功能的大量信息。比较的转录谱分析允许鉴定差异表达的基因，这些基因与成熟过程中调节果实代谢和酚含量的潜在相关性。

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