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首页> 外文期刊>BMC Genomics >Genome-wide identification, characterization and expression analysis of populus leucine-rich repeat receptor-like protein kinase genes
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Genome-wide identification, characterization and expression analysis of populus leucine-rich repeat receptor-like protein kinase genes

机译：全基因组鉴定，表征和表达分析的富胡杨亮氨酸重复受体样蛋白激酶基因

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Background Leucine-rich repeat receptor-like kinases (LRR-RLKs) comprise the largest group within the receptor-like kinase (RLK) superfamily in plants. This gene family plays critical and diverse roles in plant growth, development and stress response. Although the LRR-RLK families in Arabidopsis and rice have been previously analyzed, no comprehensive studies have been performed on this gene family in tree species. Results In this work, 379 LRR-RLK genes were retrieved from the Populus trichocarpa genome and further grouped into 14 subfamilies based on their structural and sequence similarities. Approximately 82% (312 out of 379) of the PtLRR-RLK genes are located in segmental duplication blocks indicating the role of duplication process in the expansion of this gene family. The conservation and variation in motif composition and intron/exon arrangement among PtLRR-RLK subfamilies were analyzed to provide additional support for their phylogenetic relationship and more importantly to indicate the potential divergence in their functions. Expression profiling of PtLRR-RLKs showed that they were differentially expressed in different organs and tissues and some PtLRR-RLKs were specifically expressed in meristem tissues, which indicated their potential involvement in tissue development and differentiation. For most AtLRR-RLKs with defined functions, Populus homologues exhibiting similar expression patterns could be identified, which might indicate the functional conservation during evolution. Among 12 types of environmental cues analyzed by the genome-wide microarray data, PtLRR-RLKs showed specific responses to shoot organogenesis, wounding, low ammonium feeding, hypoxia and seasonal dormancy, but not to drought, re-watering after drought, flooding, AlCl3 treatment and bacteria or fungi treatments. Conclusions This study provides the first comprehensive genomic analysis of the Populus LRR-RLK gene family. Segmental duplication contributes significantly to the expansion of this gene family. Populus and Arabidopsis LRR-RLK homologues not only share similar genetic structures but also exhibit comparable expression patterns which point to the possible functional conservation of these LRR-RLKs in two model systems. Transcriptome profiling provides the first insight into the functional divergence among PtLRR-RLK gene subfamilies and suggests that they might take important roles in growth and adaptation of tree species.
机译：背景富含亮氨酸的重复受体样激酶（LRR-RLK）构成植物中受体样激酶（RLK）超家族中的最大基团。这个基因家族在植物生长，发育和胁迫响应中起着至关重要的作用。尽管先前已经分析了拟南芥和水稻中的LRR-RLK家族，但尚未对该树种中的该基因家族进行全面研究。结果在这项工作中，从毛果杨基因组中检索到379个LRR-RLK基因，并根据其结构和序列相似性将其进一步分为14个亚科。大约82％（379个中的312个）PtLRR-RLK基因位于片段复制区中，表明复制过程在该基因家族扩展中的作用。分析了PtLRR-RLK亚家族中基序组成和内含子/外显子排列的保守性和变异性，为它们的系统发育关系提供了额外的支持，更重要的是表明了它们功能的潜在差异。 PtLRR-RLKs的表达谱显示它们在不同器官和组织中差异表达，并且某些PtLRR-RLKs在分生组织中特异性表达，表明它们可能参与组织发育和分化。对于大多数具有确定功能的AtLRR-RLK，可以鉴定出表现出相似表达模式的胡杨同源物，这可能表明进化过程中的功能保守性。在全基因组微阵列数据分析的12种环境线索中，PtLRR-RLKs显示出对芽器官发生，受伤，低铵饲喂，缺氧和季节性休眠的特异性反应，但对干旱，干旱后的补水，洪水，AlCl没有反应 3 处理以及细菌或真菌处理。结论这项研究为胡杨LRR-RLK基因家族提供了第一个全面的基因组分析。节段重复显着促进​​该基因家族的扩展。杨和拟南芥LRR-RLK同源物不仅共享相似的遗传结构，而且表现出可比的表达模式，这表明这些LRR-RLK在两个模型系统中可能的功能保守性。转录组分析提供了对PtLRR-RLK基因亚家族之间功能差异的初步了解，并表明它们可能在树木的生长和适应中发挥重要作用。

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