...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >Genome-wide identification of potato long intergenic noncoding RNAs responsive to Pectobacterium carotovorum subspecies brasiliense infection
【24h】

Genome-wide identification of potato long intergenic noncoding RNAs responsive to Pectobacterium carotovorum subspecies brasiliense infection

机译：全基因组范围内的马铃薯长的基因间非编码RNAs的响应，这些基因对巴西菜柔毛杆菌亚种感染有反应

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Long noncoding RNAs (lncRNAs) represent a class of RNA molecules that are implicated in regulation of gene expression in both mammals and plants. While much progress has been made in determining the biological functions of lncRNAs in mammals, the functional roles of lncRNAs in plants are still poorly understood. Specifically, the roles of long intergenic nocoding RNAs (lincRNAs) in plant defence responses are yet to be fully explored. Results In this study, we used strand-specific RNA sequencing to identify 1113 lincRNAs in potato ( Solanum tuberosum ) from stem tissues. The lincRNAs are expressed from all 12 potato chromosomes and generally smaller in size compared to protein-coding genes. Like in other plants, most potato lincRNAs possess single exons. A time-course RNA-seq analysis between a tolerant and a susceptible potato cultivar showed that 559 lincRNAs are responsive to Pectobacterium carotovorum subsp. brasiliense challenge compared to mock-inoculated controls. Moreover, coexpression analysis revealed that 17 of these lincRNAs are highly associated with 12 potato defence-related genes. Conclusions Together, these results suggest that lincRNAs have potential functional roles in potato defence responses. Furthermore, this work provides the first library of potato lincRNAs and a set of novel lincRNAs implicated in potato defences against P. carotovorum subsp. brasiliense , a member of the soft rot Enterobacteriaceae phytopathogens.
机译：背景技术长非编码RNA（lncRNA）代表一类RNA分子，与哺乳动物和植物的基因表达调控有关。尽管在确定哺乳动物中lncRNA的生物学功能方面已取得了很大进展，但对lncRNA在植物中的功能作用仍知之甚少。具体而言，长基因间无编码RNA（lincRNA）在植物防御反应中的作用尚待充分研究。结果在这项研究中，我们使用链特异性RNA测序从茎组织中鉴定马铃薯（马铃薯）的1113个lincRNA。 lincRNAs从所有12个马铃薯染色体上表达，与蛋白质编码基因相比，通常大小较小。像其他植物一样，大多数马铃薯lincRNA具有单个外显子。在耐性和易感马铃薯品种之间进行的时程RNA-seq分析表明，有559个inclRNA对胡萝卜果孢菌亚种有反应。与模拟接种的控件相比，brasiliense挑战。此外，共表达分析表明，这些lincRNA中有17个与12个马铃薯防御相关基因高度相关。结论总之，这些结果表明lincRNA在马铃薯防御反应中具有潜在的功能作用。此外，这项工作提供了马铃薯lincRNA的第一个文库和一组涉及马铃薯对胡萝卜腐烂假单胞菌防御的新型lincRNA。 brasiliense，软腐肠杆菌科植物病原体的成员。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号