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首页> 外文期刊>BMC Genomics >RNA-sequencing elucidates the regulation of behavioural transitions associated with the mating process in honey bee queens
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RNA-sequencing elucidates the regulation of behavioural transitions associated with the mating process in honey bee queens

机译：RNA测序阐明了与蜂皇后交配过程相关的行为转变的调控

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Mating is a complex process, which is frequently associated with behavioural and physiological changes. However, understanding of the genetic underpinnings of these changes is limited. Honey bees are both a model system in behavioural genomics, and the dominant managed pollinator of human crops; consequently understanding the mating process has both pure and applied value. We used next-generation transcriptomics to probe changes in gene expression in the brains of honey bee queens, as they transition from virgin to mated reproductive status. In addition, we used CO2-narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation. The mating process produced significant changes in the expression of vision, chemo-reception, metabolic, and immune-related genes. Differential expression of these genes maps clearly onto known behavioural and physiological changes that occur during the transition from being a virgin queen to a newly-mated queen. A subset of these changes in gene expression were also detected in CO2-treated queens, as predicted from previous physiological studies. In addition, we compared our results to previous studies that used microarray techniques across a range of experimental time-points. Changes in expression of immune- and vision-related genes were common to all studies, supporting an involvement of these groups of genes in the mating process. Our study is an important step in understanding the molecular mechanisms regulating post-mating behavioural transitions in a natural system. The weak overlap in patterns of gene expression with previous studies demonstrates the high sensitivity of genome-wide approaches. Thus, while we build on previous microarray studies that explored post-mating changes in honey bees, the broader experimental design, use of RNA-sequencing, and focus on Australian honey bees, which remain free from the devastating parasite Varroa destructor, in the current study, provide unique insights into the biology of the mating process in honey bees.
机译：交配是一个复杂的过程，通常与行为和生理变化有关。但是，对这些变化的遗传基础的理解是有限的。蜜蜂既是行为基因组学的模型系统，也是人类作物的主要管理传粉媒介。因此，了解交配过程具有纯价值和应用价值。我们使用了下一代转录组学技术来研究蜂皇后大脑中基因表达的变化，因为它们从原始状态转变为繁殖状态。此外，我们使用了CO2麻醉，它可以在不交配的情况下诱导产卵，从而隔离生殖成熟过程。交配过程在视觉，化学接受，代谢和免疫相关基因的表达上产生了重大变化。这些基因的差异表达清楚地映射到已知的行为和生理变化，这些变化在从原始女王/王后过渡到新女王/王后的过程中发生。正如先前的生理研究所预测的那样，在经CO2处理的皇后区中也检测到了这些基因表达变化的子集。此外，我们将我们的研究结果与以前在一系列实验时间点上使用微阵列技术的研究进行了比较。免疫和视觉相关基因表达的变化在所有研究中都是常见的，从而支持了这些基因组在交配过程中的参与。我们的研究是了解调节自然系统中交配后行为转变的分子机制的重要步骤。基因表达模式与以前的研究之间的弱重叠证明了全基因组方法的高度敏感性。因此，尽管我们以以前的微阵列研究为基础，探索了蜜蜂交配后的变化，但目前的实验设计更广泛，RNA测序的应用以及专注于澳大利亚蜜蜂，而澳大利亚蜜蜂目前仍没有破坏性的寄生虫Varroa破坏者研究提供了蜜蜂交配过程生物学的独特见解。

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