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首页> 外文期刊>British Journal of Cancer >Spontaneous and chemically induced transformation of rat embryo cell cultures
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Spontaneous and chemically induced transformation of rat embryo cell cultures

机译：大鼠胚胎细胞培养的自发和化学诱导转化

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The transformation of Wistar rat embryo cells in vitro has been studied in passaged cultures using two criteria for transformation: (1) the ability of cells to form colonies in soft agar and (2) the ability of cells to form tumours in young syngeneic animals. In general there was good correlation between the two criteria. Spontaneous transformation was observed in all untreated cultures by 44 weeks although, by not allowing the cells to become confluent, the tendency was for cultures to transform earlier (i.e. 15-21 weeks). It was noticeable that despite untreated cultures having been in vitro for different lengths of time, most cultures transformed after a similar number of passages (42-50). Treatment of the embryo cells with the alkylating agent nitrosomethylurea (NMU) or benzo(alpha)pyrene (BP) sometimes resulted in transformation after a shorter period in vitro than the controls (minimum 12 weeks) although some treated cultures took longer. Transformed cells produced transplantable fibrosarcomata in syngeneic hosts and those arising from NMU transformed cells were histologically different from those arising from spontaneously transformed cells. The significant of spontaneous transformation in in vitro rat cell transformation systems is discussed.
机译：已经在传代培养中使用两种转化标准对Wistar大鼠胚胎细胞的体外转化进行了研究：（1）细胞在软琼脂中形成菌落的能力;（2）在年轻的同系动物中细胞形成肿瘤的能力。通常，两个标准之间具有良好的相关性。在44周之前，在所有未处理的培养物中都观察到了自发转化，尽管由于不允许细胞融合而使培养物更早转化（即15-21周）的趋势。值得注意的是，尽管未经处理的培养物已在体外培养了不同的时间长度，但大多数培养物在经过相似的传代次数后仍发生了转化（42-50）。用烷基化剂亚硝基甲基脲（NMU）或苯并αpy（BP）处理胚胎细胞有时会在体外比对照短的时间（至少12周）后导致转化，尽管某些处理的培养时间更长。转化细胞在同系宿主中产生可移植的纤维肉瘤，而由NMU转化细胞产生的细胞在组织学上与自发转化细胞产生的组织学不同。讨论了体外大鼠细胞转化系统中自发转化的意义。

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